IRF-3-dependent and augmented target genes during viral infection
IRF-3-dependent and augmented target genes during viral infection
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DOI：
10.1038/sj.gene.6364449
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发表时间：
2008-03-01
影响因子：
5
通讯作者：
Reich, N. C.
中科院分区：
文献类型：
--
作者：
Andersen, J.;VanScoy, S.;Reich, N. C.
转录因子干扰素调节因子 - 3（IRF - 3）的激活是对病毒感染的先天免疫应答中的一个关键事件。为了解IRF - 3对宿主防御的作用，我们采用系统生物学方法分析依赖于IRF - 3的全局基因表达。对病毒感染后IRF - 3基因敲除动物细胞或野生型同胞细胞的表达谱进行比较，揭示了三组诱导基因，即严格依赖于IRF - 3的基因、因IRF - 3而增强表达的基因以及对IRF - 3无反应的基因。已鉴定的IRF - 3靶基因产物参与先天或后天免疫，或参与细胞周期、细胞凋亡和增殖的调控。这些结果揭示了一种转录因子在免疫应答中的全局效应，并为评估对病毒感染的综合应答提供了信息。
Activation of the transcription factor interferon regulatory factor-3 (IRF-3) is an essential event in the innate immune response to viral infection. To understand the contribution of IRF-3 to host defense, we used a systems biology approach to analyze global gene expression dependent on IRF-3. Comparison of expression profiles in cells from IRF-3 knockout animals or wild-type siblings following viral infection revealed three sets of induced genes, those that are strictly dependent on IRF-3, augmented with IRF-3, or not responsive to IRF-3. Products of identified IRF-3 target genes are involved in innate or acquired immunity, or in the regulation of cell cycle, apoptosis and proliferation. These results reveal the global effects of one transcription factor in the immune response and provide information to evaluate the integrated response to viral infection.