A pharmacological study on respiratory rhythm in the isolated brainstem‐spinal cord preparation of the newborn rat
A pharmacological study on respiratory rhythm in the isolated brainstem‐spinal cord preparation of the newborn rat
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新生大鼠离体脑干脊髓制剂呼吸节律的药理学研究
DOI：
10.1111/j.1476-5381.1985.tb09439.x
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发表时间：
1985
影响因子：
7.3
通讯作者：
S. Tamai
中科院分区：
文献类型：
--
作者：
T. Murakoshi;T. Suzue;S. Tamai
1采用新生大鼠体外脑干-脊髓标本，观察神经递质和候选递质对呼吸频率的影响。2以5~15min的−1频率持续5h以上，观察到4、5颈段脊髓腹根自发性周期性去极化，以此去极化频率作为呼吸频率的指标。3钙、镁离子浓度升高，呼吸频率降低，K+浓度升高，呼吸频率增加。这种频率也随着pH的降低而增加。4多巴胺、5-羟色胺、组胺、乙酰胆碱、谷氨酸、P物质和促甲状腺激素释放激素在脑干灌流时加快呼吸频率，而去甲肾上腺素、γ-氨基丁酸、甘氨酸、脑啡肽和亮氨酸脑啡肽使呼吸频率减慢。拮抗剂实验表明，乙酰胆碱对呼吸频率的刺激作用主要由M受体介导，去甲肾上腺素的抑制作用主要由α-肾上腺素受体介导。
1 An in vitro brainstem‐spinal cord preparation of the newborn rat was used to examine the effects of neurotransmitters and transmitter candidates on respiratory frequency. 2 Spontaneous periodic depolarization of the spinal ventral roots of the 4th or 5th cervical segment was observed at a frequency of 5–15 min−1 constantly for more than 5 h. The frequency of this depolarization was monitored as an index of the respiratory frequency. 3 An elevation of the concentration of Ca2+ or Mg2+ caused a decrease in the respiratory frequency, whereas an elevation of K+ concentration caused an increase. The frequency was also increased by a reduction of pH. The highest frequency was observed at 27–28°C. 4 Dopamine, 5‐hydroxytryptamine, histamine, acetylcholine, glutamic acid, substance P, and thyrotropin releasing hormone accelerated the respiratory frequency when applied by perfusion to the brainstem, whereas noradrenaline, γ‐aminobutyric acid, glycine, and [Met5] enkephalin and [Leu5] enkephalin slowed the frequency. Experiments with antagonists suggested that the stimulant effect of acetylcholine on respiratory frequency was mediated mainly by muscarinic receptors and the depressant effect of noradrenaline was mediated by α‐adrenoceptors.
呼吸控制的神经药理学。
影响因子：
21.1
作者：
Mueller,RA;Lundberg,DB;Breese,GR;Hedner,J;Hedner,T;Jonason,J
通讯作者：
Jonason,J