Direct observation of unstained biological samples in water using newly developed impedance scanning electron microscopy
Direct observation of unstained biological samples in water using newly developed impedance scanning electron microscopy
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DOI：
10.1371/journal.pone.0221296
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发表时间：
2019-08-20
期刊：
影响因子：
3.7
通讯作者：
Ogura, Toshihiko
中科院分区：
文献类型：
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作者：
Ogura, Toshihiko
在生物学、化学、材料科学和纳米技术等科学领域中，对水中样品进行纳米尺度的观测是必不可少的。扫描电子显微镜（SEM）在高真空条件下获得生物样品的高分辨率图像，但需要特定的样品制备方案。观察水中未染色的生物样品需要更方便和侵入性更小的方法。在这里，我们已经开发了一种新型的阻抗显微镜，即阻抗扫描电镜（IP-SEM），它允许成像和亚微米尺度的各种标本在水中的检查。通过改变输入信号的频率，所提出的系统可以在亚微米尺度分辨率下检测样品的成分的阻抗特性。除了检查水中各种未染色的生物标本和材料样品外。此外，所提出的系统可用于广泛的科学领域的各种液体样品，如纳米颗粒，纳米管和有机和催化材料。
Nanometre-scale observation of specimens in water is indispensable in several scientific fields, such as biology, chemistry, materials science and nanotechnology. Scanning electron microscopy (SEM) obtains high-resolution images of biological samples under high vacuum conditions but requires specific sample-preparation protocols. Observations of unstained biological samples in water require more convenient and less invasive methods. Herein, we have developed a new type of impedance microscopy, namely impedance SEM (IP-SEM), which allows the imaging and sub-micrometer scale examination of various specimens in water. By varying the frequency of the input signal, the proposed system can detect the impedance properties of the sample's composition at sub-micrometer scale resolution. Besides examining various unstained biological specimens and material samples in water. Furthermore, the proposed system can be used for diverse liquid samples across a broad range of scientific fields, such as nanoparticles, nanotubes and organic and catalytic materials.