DIFFERENTIAL IN-SITU CYTOKINE GENE-EXPRESSION IN ACUTE VERSUS CHRONIC ATONIC DERMATITIS
DIFFERENTIAL IN-SITU CYTOKINE GENE-EXPRESSION IN ACUTE VERSUS CHRONIC ATONIC DERMATITIS
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DOI：
10.1172/jci117408
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发表时间：
1994-08-01
影响因子：
15.9
通讯作者：
LEUNG, DYM
中科院分区：
文献类型：
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作者：
HAMID, Q;BOGUNIEWICZ, M;LEUNG, DYM
特应性皮炎（AD）皮肤炎症的发生和维持机制尚不清楚。最近的数据表明，局部表达的细胞因子的模式在调节组织炎症的性质中起着关键作用。在这项研究中，我们使用原位杂交研究白细胞介素4（IL-4），IL-5，和干扰素-γ（IFN-γ）信使RNA（mRNA）的表达在皮肤活检从急性和慢性皮肤病变的AD患者。与正常对照皮肤或AD患者的未受累皮肤相比，急、慢性皮损中表达IFN-γ mRNA、IL-4（P < 0.01）和IL-5（P < 0.01）的细胞数明显增多，但表达IFN-γ mRNA的细胞数无明显变化。而慢性AD皮损中IL-4 mRNA表达细胞数明显少于急性AD皮损（P < 0.01），而IL-5 mRNA表达细胞数明显高于急性AD皮损（P < 0.01）。在急性和慢性AD病变中，T细胞构成了大多数IL-5表达细胞。慢性病变组EG 2+嗜酸性粒细胞的表达明显高于急性病变组（P < 0.01）。这些数据表明，虽然急性和慢性AD病变与IL-4和IL-5基因的活化增加有关，但AD中急性皮肤炎症的起始与IL-4表达的优势有关，而慢性炎症的维持主要与IL-5表达增加和嗜酸性粒细胞浸润有关。
The mechanisms involved in the initiation and maintenance of skin inflammation in atopic dermatitis (AD) are poorly understood. Recent data suggest that the pattern of cytokines expressed locally plays a critical role in modulating the nature of tissue inflammation. In this study, we used in situ hybridization to investigate the expression of interleukin 4 (IL-4), IL-5, and interferon-gamma (IFN-gamma) messenger RNA (mRNA) in skin biopsies from acute and chronic skin lesions of patients with AD. As compared with normal control skin or uninvolved skin of patients with AD, acute and chronic skin lesions had significantly greater numbers of cells that were positive for mRNA, IL-4 (P < 0.01), and IL-5 (P < 0.01), but not for IFN-gamma mRNA expressing cells. However, as compared with acute AD skin lesions, chronic AD skin lesions had significantly fewer IL-4 mRNA-expressing cells (P < 0.01), but significantly greater IL-5 mRNA (P < 0.01). T cells constituted the majority of IL-5-expressing cells in acute and chronic AD lesions. Chronic lesions also expressed significantly greater numbers of activated EG2+ eosinophils than acute lesions (P < 0.01). These data indicate that although acute and chronic AD lesions are associated with increased activation of IL-4 and IL-5 genes, initiation of acute skin inflammation in AD is associated with a predominance of IL-4 expression whereas maintenance of chronic inflammation is predominantly associated with increased IL-5 expression and eosinophil infiltration.