Expression of the largest CD97 and EMR2 isoforms on leukocytes facilitates a specific interaction with chondroitin sulfate on B cells
Expression of the largest CD97 and EMR2 isoforms on leukocytes facilitates a specific interaction with chondroitin sulfate on B cells
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DOI：
10.1189/jlb.0704402
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发表时间：
2005-01-01
影响因子：
5.5
通讯作者：
Hamann, J
中科院分区：
文献类型：
--
作者：
Kwakkenbos, MJ;Pouwels, W;Hamann, J
EGF-TM 7受体CD 97和EMR 2是主要在白细胞上表达的七螺旋分子。这些受体的一个特征是它们通过N-末端表皮生长因子（EGF）样结构域与细胞配体相互作用的能力。CD 97的前两个EGF结构域（而不是EMR 2）结合CD 55（衰变加速因子），而CD 97和EMR 2的第四个EGF结构域与糖胺聚糖硫酸软骨素（CS）相互作用。使用包被有可溶性重组CD 97和EMR 2蛋白的荧光珠和同种型特异性单克隆抗体，我们已经确定了与CS相互作用的细胞和分子特征。CD 97和EMR 2的第四EGF结构域在活化的淋巴细胞和粘液样细胞上表达，而配体特异性地在外周血内的B细胞上发现。因此，CD 97/EMR 2和CS之间的相互作用可能在活化的T细胞、树突状细胞和巨噬细胞与B细胞的相互作用中发挥作用。
The EGF-TM7 receptors CD97 and EMR2 are heptahelical molecules predominantly expressed on leukocytes. A characteristic of these receptors is their ability to interact with cellular ligands via the N-terminal epidermal growth factor (EGF)-like domains. The first two EGF domains of CD97 (but not EMR2) bind CD55 (decay-accelerating factor), while the fourth EGF domain of both CD97 and EMR2 interacts with the glycosaminoglycan chondroitin sulfate (CS). Using fluorescent beads coated with soluble recombinant CD97 and EMR2 protein, and isoform-specific monoclonal antibodies, we have determined the cellular and molecular characteristics of the interaction with CS. The fourth EGF domain of CD97 and EMR2 is expressed on activated lymphocytes and mycloid cells, whereas the ligand is specifically found on B cells within the peripheral blood. The interaction between CD97/EMR2 and CS may therefore play a role in the interaction of activated T cells, dendritic cells, and macrophages with B cells.