Chemical Studies on Different Color Development in Blue- and Red-Colored Sepal Cells of Hydrangea macrophylla
Chemical Studies on Different Color Development in Blue- and Red-Colored Sepal Cells of Hydrangea macrophylla
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DOI：
10.1271/bbb.80831
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发表时间：
2009-05-01
影响因子：
1.6
通讯作者：
Yoshida, Kumi
中科院分区：
文献类型：
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作者：
Ito, Daisuke;Shinkai, Yosuke;Yoshida, Kumi
为了阐明绣球花瓣蓝色和红色发育差异的原因，我们对染色细胞中的有机和无机成分进行了分析。为获得有色原生质体，分别用纤维素酶和果胶酶联合处理每个蓝色和红色的萼片组织，然后用微吸管从有色和无色原生质体混合物的悬液中收集相同色调的有色细胞。采用半微量高效液相色谱法和石墨炉原子吸收光谱分析技术，分别对染色细胞中的有机成分(飞雀素3-葡萄糖苷、绿原酸、新绿原酸和5-O-对香豆酰奎宁酸)和Al3+的含量进行了测定。在蓝色单元格中，13eq.5-O-酰基奎宁酸和1.2eq.Al~(3+)对花色苷的影响较大。与此结果相反，在红细胞中，只有3.6eq。5-O-酰基奎宁酸和0.03eq.Al~(3+)。通过将这些成分混合在一起对每种蓝色和红色的萼片颜色进行复制实验，得出结论：对于蓝色着色，5-O-酰基奎宁酸和Al3+都是必不可少的。
To clarify the cause of the difference in blue and red color development of hydrangea sepals, Hydrangea macrophylla, we analyzed the organic and inorganic components in the colored cells. To obtain colored protoplasts, each blue and red sepal tissue was treated With a combination of cellulase and pectinase, and then from the suspension of the olored and colorless protoplast mixture colored cells of the same hue were collected with a micro-pipette. The content of organic components (delphinidin 3-glucoside, chlorogenic acid, neochlorogenic acid and 5-O-p-coumaroylquinic acid) and Al3+ in each colored cell was quantified respectively by semimicro-HPLC and graphite furnace atomic absorption spectroscopy (GFAAS). In the blue cells 13 eq. of 5-O-acylquinic acids and 1.2 eq. of Al3+ to anthocyanin were contained. Contrary to this result, in the red cells, only 3.6 eq. of 5-O-acylquinic acids and 0.03 eq. of Al3+ were detected. A reproduction experiment of each blue and red sepal color by mixing those components concluded that, for blue coloration, both 5-O-acylquinic acids and Al3+ were essential.