Non-conservative mutations are well tolerated in the globular region of yeast histone H4.
Non-conservative mutations are well tolerated in the globular region of yeast histone H4.
复制标题
酵母组蛋白 H4 的球状区域对非保守突变具有良好的耐受性。
DOI：
10.1006/jmbi.1996.0033
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Behe,MJ
中科院分区：
文献类型：
--
作者：
Agarwal,S;Behe,MJ
酵母组蛋白H4在参与核小体球状核心的几个位置被诱变。无论是酵母、四膜虫还是更高级的真核生物，天然蛋白在所有已知的H4序列中都含有不变或高度保守的残基。尽管如此，这种蛋白质对非保守性突变具有耐受性。在细胞功能水平上，突变蛋白对交配效率的细胞周期长度没有显著影响。在染色质结构水平上，未观察到对染色质间间距或核小体DNA羟基自由基切割模式的影响。
Yeast histone H4 has been mutagenized at several positions which participate in the globular core of the nucleosome. The native protein contains residues at those positions which are invariant or highly conserved over all known H4 sequences, whether from yeast,Tetrahymenaor higher eukaryotes. Nonetheless the protein is tolerant of non-conservative mutations. At the level of cell function the mutant proteins cause no significant change in length of the cell cycle of mating efficiency. At the level of chromatin structure no effect is observed on the internucleosomal spacing of chromatin or the pattern of hydroxyl radical cleavage of nucleosomal DNA.