Gonadotropin-releasing hormone and gonadotropin-releasing hormone receptor messenger ribonucleic acids expression in nontumorous and neoplastic pituitaries
Gonadotropin-releasing hormone and gonadotropin-releasing hormone receptor messenger ribonucleic acids expression in nontumorous and neoplastic pituitaries
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DOI：
10.1210/jc.82.6.1974
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发表时间：
1997-06-01
影响因子：
5.8
通讯作者：
Lloyd, RV
中科院分区：
文献类型：
--
作者：
Sanno, N;Jin, L;Lloyd, RV
在非肿瘤性垂体中，GnRH通过GnRH受体(GnRH-R)刺激促性腺激素细胞释放和合成黄体生成素和卵泡刺激素。然而，关于GnRH和GnRH-R信使RNAs(MRNAs)在非肿瘤性垂体组织和腺瘤中的表达知之甚少。为了进一步了解GnRH及其受体的分布和调节作用，我们采用RT-PCR、原位杂交和原位杂交结合RT-PCR技术，研究了GnRH和GnRH-RmRNAs在非肿瘤性垂体和不同类型的垂体腺瘤中的表达。RT-PCR检测发现，GnRH mRNA在正常脑垂体和各种类型的腺瘤中均有表达。类似地，GnRH-R mRNA在非肿瘤性垂体和大多数(但不是全部)腺瘤中表达。其中促性腺激素腺瘤5例，零细胞腺瘤6例，GH腺瘤1例，ACTH腺瘤1例，但2例PRL腺瘤中均无。原位杂交显示GnRH和GnRH-R在3例非肿瘤性垂体瘤和33例腺瘤中分别为12例(36.4%)和10例(30.3%)。采用间接原位RT-PCR技术提高原位定位的敏感性，在33例腺瘤中分别检测到29例(87.9%)和25例(75.8%)GnRH和GnRH-RmRNAs，这是首次应用原位RT-PCR技术在单个垂体腺瘤细胞中定位GnRH和GnRH-RmRNAs。GnRH和GnRH-RmRNAs在垂体细胞中频繁表达，提示GnRH在非肿瘤和肿瘤组织中具有自分泌/旁分泌功能。
In the nontumorous pituitary, GnRH stimulates the release and synthesis of LH and FSH by gonadotroph cells via the GnRH receptor (GnRH-R). Little is known, however, about expression of GnRH and GnRH-R messenger RNAs (mRNAs) in nontumorous pituitary tissue and in adenomas. To learn more about the distribution and regulatory roles of GnRH and its receptor, we investigated the expression of both GnRH and GnRH-R mRNAs in nontumorous human pituitary and in various types of pituitary adenomas using the RT-PCR, in situ hybridization, and in situ hybridization in combination with RT-PCR (in situ RT-PCR). Using RT-PCR, GnRH mRNA was found to be expressed in normal human pituitaries and in all types of adenomas. Similarly, GnRH-R mRNA was expressed in nontumorous human pituitaries and in most, but not all, adenomas. These included 5 gonadotroph adenomas, 6 null cell adenomas, 1 of 2 GH-producing tumors, and 1 of 2 ACTH-producing adenomas, but not in the 2 PRL-producing adenomas examined. In situ hybridization studies showed GnRH and GnRH-R mRNAs in all 3 nontumorous pituitaries and in 12 of 33 (36.4%) and 10 of 33 adenomas (30.3%), respectively. Using an indirect in situ RT-PCR technique to increase the sensitivity of the in situ localization, GnRH and GnRH-R mRNAs were detected in 29 (87.9%) and 25 (75.8%) of 33 adenomas, respectively.This is the first report of the localization of GnRH and GnRH-R mRNAs in individual pituitary adenoma cells using in situ RT-PCR. The frequent expression of GnRH and GnRH-R mRNAs in pituitary cells suggests that GnRH has autocrine/paracrine functions in nontumorous and neoplastic pituitary tissues.