Expression of SpC3, the sea urchin complement component, in response to lipopolysaccharide
Expression of SpC3, the sea urchin complement component, in response to lipopolysaccharide
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DOI：
10.1007/s002510000233
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发表时间：
2000-10-01
期刊：
影响因子：
3.2
通讯作者：
Smith, LC
中科院分区：
文献类型：
--
作者：
Clow, LA;Gross, PS;Smith, LC
脊椎动物补体成分C3的同源物中表达的体腔细胞的紫海胆，球海胆purpuratus，指定SpC 3，进行了研究的变化，在响应免疫挑战或损伤。在本研究中使用免疫静止动物，因为它们的体腔细胞或体腔液（CF）中具有减少的或不可检测的SpC 3。动物注射脂多糖（LPS）或无菌海水（SSW，损伤对照）。然后通过蛋白质印迹和ELISA随时间跟踪体腔液和体腔细胞中SpC 3量的变化。还通过RT-PCR跟踪来自SpC 3基因（Sp 064）的mRNA的变化。虽然所有动物对损伤的反应都是体腔液中SpC 3水平升高，但LPS激发的动物CF和体腔细胞中SpC 3的含量均高于接受SSW的动物。在大多数接受LPS的动物中，在注射后1小时内观察到SpC 3的初始增加，而接受SSW的动物中的最早反应为6小时。SpC 3在体腔细胞中的出现比其在CF中的出现延迟，并且在攻击后几天首次检测到。Sp 064基因mRNA的变化与SpC 3在体腔液中的出现有关。每毫升CF的体腔细胞数量和SpC 3（+）体腔细胞百分比的增加也发生在LPS激发后或对损伤的反应中，对LPS的反应增加略大。虽然SpC 3的变化没有以前确定的巨噬细胞中表达的人C3那么大，但反应的动力学与哺乳动物中的急性期反应物相似。
The homologue of the vertebrate complement component C3 that is expressed in the coelomocytes of the purple sea urchin, Strongylocentrotus purpuratus, designated SpC3, was investigated for changes in response to immune challenge or injury. Immunoquiescent animals were used in this study because they have reduced or no detectible SpC3 in their coelomocytes or coelomic fluid (CF). Animals were injected with lipopolysaccharide (LPS) or sterile sea water (SSW, injury control). Changes in the amounts of SpC3 in coelomic fluid and in coelomocytes were then followed over time by Western blots and ELISA. Changes in mRNA from the SpC3 gene (Sp064) were also followed by RT-PCR. Although all animals responded to injury with increased levels of SpC3 in the coelomic fluid, those challenged with LPS had greater amounts of SpC3 in both CF and coelomocytes than those receiving SSW. In most of the animals receiving LPS, initial increases in SpC3 were observed within 1 h post-injection, while the earliest response in the animals receiving SSW was 6 h. The appearance of SpC3 in the coelomocytes was delayed compared to its appearance in CF, and was first detected several days after challenge. Changes in mRNA from the Sp064 gene paralleled the appearance of SpC3 in the coelomic fluid. Increases in the number of coelomocytes per milliliter of CF and in the percentage of coelomocytes that were SpC3(+) also occurred after challenge with LPS or in response to injury, with a slightly greater increase in response to LPS. Although the changes in SpC3 were not as great as those identified previously for human C3 expressed in macrophages, the kinetics of the response are similar to that of acute-phase reactants in mammals.