Inhibition Performance of Lignocellulose Degradation Products on Industrial Cellulase Enzymes During Cellulose Hydrolysis
Inhibition Performance of Lignocellulose Degradation Products on Industrial Cellulase Enzymes During Cellulose Hydrolysis
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DOI：
10.1007/s12010-009-8525-z
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发表时间：
2009-12-01
影响因子：
3
通讯作者：
Bao, Jie
中科院分区：
文献类型：
--
作者：
Jing, Xinyun;Zhang, Xiaoxi;Bao, Jie
本研究考察了主要木质纤维素降解产物（包括有机酸、呋喃衍生物、木质素衍生物和乙醇）对广泛使用的商业纤维素酶Spezyme CP（Genencor International，罗切斯特，NY，USA）在充分混合状态（摇瓶）和静态（试管）下对纤维素水解的抑制性能。将Spezyme CP的纤维素酶复合物中的纤维素酶活性假定为单一的“纤维素酶”，并测定了该纤维素酶的表观动力学参数，作为对工业纤维素酶抑制效果的近似指标。利用测定的表观动力学参数对降解产物的缓蚀性能进行了比较和分析。各降解产物对纤维素酶水解纤维素均有强烈的抑制作用，且均为竞争性抑制。木质纤维素降解产物对纤维素酶的抑制作用大小顺序为木质素衍生物>呋喃衍生物>有机酸>乙醇。本研究定量地考察了木质纤维素酶解过程中木质纤维素降解产物的抑制性能，有助于了解木质纤维素对酶解的抑制作用。
This study examined the inhibition performance by the major lignocellulose degradation products, including organic acids, furan derivatives, lignin derivatives, and ethanol, on a broadly used commercial cellulase enzyme Spezyme CP (Genencor International, Rochester, NY, USA) to cellulose hydrolysis at both the well-mixing state (shaking flask) and the static state (test tube). The cellulase activity in the cellulase complex of Spezyme CP was assumed to be one single "cellulase", and the apparent kinetic parameters of this cellulase enzyme were measured as an approximate index of the inhibitory effect to the industrial cellulase enzyme. The inhibition performance of these degradation products was compared and analyzed using the determined apparent kinetic parameters. All the degradation products strongly inhibit the cellulose hydrolysis by cellulase enzyme, and the inhibitions on cellulase were all competitive type. The order of the inhibition strength by the lignocellulose degradation products to cellulase is lignin derivatives > furan derivatives > organic acids > ethanol. This study gave a quantitative view to the enzymatic hydrolysis of lignocellulose under the inhibition performance of the lignocellulose degradation products and will help to understand the lignocellulose recalcitrance to enzyme hydrolysis.