Drosophila Unfolded Protein Response (UPR) Assays In Vitro and In Vivo.
Drosophila Unfolded Protein Response (UPR) Assays In Vitro and In Vivo.
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DOI：
10.1007/978-1-0716-1732-8_17
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发表时间：
2022
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表达超出细胞蛋白质折叠系统能力的野生型或突变蛋白可能不利于一般细胞功能和生存。为了应对内质网 (ER) 中错误折叠的蛋白质超载，真核细胞激活未折叠蛋白质反应 (UPR)，帮助细胞恢复内质网 (ER) 中的蛋白质稳态。作为 UPR 的一部分，细胞减弱一般 mRNA 翻译并激活诱导应激反应基因表达的转录因子。 UPR 信号引起了研究兴趣，部分原因是导致 ER 中慢性蛋白质错误折叠的疾病或损害 UPR 信号的疾病是神经退行性疾病、糖尿病和癌症等多种疾病的基础。模型生物在该领域经常使用，因为 UPR 途径通常在整个门中得到很好的保存。在这里，我们介绍检测果蝇 UPR 的实验程序。
Wild type or mutant proteins expressed beyond the capacity of a cell’s protein folding system could be detrimental to general cellular function and survival. In response to misfolded protein overload in the endoplasmic reticulum (ER), eukaryotic cells activate the Unfolded Protein Response (UPR) that helps cells restore protein homeostasis in the endoplasmic reticulum (ER). As part of the UPR, cells attenuate general mRNA translation and activate transcription factors that induce stress-responsive gene expression. UPR signaling draws research interest in part because conditions that cause chronic protein misfolding in the ER, or those that impair UPR signaling underlie several diseases including neurodegeneration, diabetes and cancers. Model organisms are frequently employed in the field as the UPR pathways are generally well-conserved throughout phyla. Here, we introduce experimental procedures to detect UPR in Drosophila melanogaster.