CLONING THE PLASMODIUM-FALCIPARUM GENE ENCODING PFEMP1, A MALARIAL VARIANT ANTIGEN AND ADHERENCE RECEPTOR ON THE SURFACE OF PARASITIZED HUMAN ERYTHROCYTES
CLONING THE PLASMODIUM-FALCIPARUM GENE ENCODING PFEMP1, A MALARIAL VARIANT ANTIGEN AND ADHERENCE RECEPTOR ON THE SURFACE OF PARASITIZED HUMAN ERYTHROCYTES
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DOI：
10.1016/0092-8674(95)90054-3
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发表时间：
1995-07-14
期刊：
影响因子：
64.5
通讯作者：
HOWARD, RJ
中科院分区：
文献类型：
--
作者：
BARUCH, DI;PASLOSKE, BL;HOWARD, RJ
恶性疟原虫感染的人红细胞通过表达细胞表面变异抗原和粘附于内皮细胞的受体逃避宿主免疫。这些特性归因于恶性疟原虫红细胞膜蛋白1（PfEMP 1），其是寄生红细胞（PE）表面上的200-350 kDa的抗原多样性疟疾蛋白。我们描述了两个相关的PfEMP 1基因的克隆从马来营（MC）寄生虫株。针对基因的重组蛋白片段产生的抗体对MC菌株PfEMP 1蛋白具有特异性。这些抗体仅与MC株PE的表面反应，并阻断这些细胞粘附于CD 36，但对粘附于血小板反应蛋白没有影响。PfEMP 1基因的多种形式在MC寄生虫中是明显的。疟疾抗原变异和受感染红细胞粘附于宿主细胞的分子基础现在可以继续研究。
Plasmodium falciparum-infected human erythrocytes evade host immunity by expression of a cell-surface variant antigen and receptors for adherence to endothelial cells. These properties have been ascribed to P. falciparum erythrocyte membrane protein 1 (PfEMP1), an antigenically diverse malarial protein of 200-350 kDa on the surface of parasitized erythrocytes (PEs). We describe the cloning of two related PfEMP1 genes from the Malayan Camp (MC) parasite strain. Antibodies generated against recombinant protein fragments of the genes were specific for MC strain PfEMP1 protein. These antibodies reacted only with the surface of MC strain PEs and blocked adherence of these cells to CD36 but without effect on adherence to thrombospondin. Multiple forms of the PfEMP1 gene are apparent in MC parasites. The molecular basis for antigenic variation in malaria and adherence of infected erythrocytes to host cells can now be pursued.