Exposure of different bacterial inocula to newborn chicken affects gut microbiota development and ileum gene expression
Exposure of different bacterial inocula to newborn chicken affects gut microbiota development and ileum gene expression
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DOI：
10.1038/ismej.2009.128
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发表时间：
2010-03
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影响因子：
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通讯作者：
Yeshi Yin;Fang Lei;Liying Zhu;Sujuan Li;Zuowei Wu;Ruifen Zhang;G. Gao;B. Zhu;Xin Wang
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文献类型：
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作者：
Yeshi Yin;Fang Lei;Liying Zhu;Sujuan Li;Zuowei Wu;Ruifen Zhang;G. Gao;B. Zhu;Xin Wang
新生儿从无菌肠道环境到微生物群发育的转变尚未完全了解。本研究的目的是调查暴露于细菌群落对新孵化的鸡肠道微生物群发育的影响。将90只孵化后的雏鸡分为三组。A组和B组用盲肠来源的接种物处理，而C组用无菌水喂养。A组接种物Ⅰ的主要菌种为拟杆菌（20.7%）、毛螺菌（17.2%）和未分类的瘤胃球菌（16.1%）; B组接种物Ⅱ的主要菌种为普雷沃氏菌（37.9%）、氨基酸球菌（16.1%）和多雷氏菌（12.6%）。对15天内回肠和盲肠内容物的分析表明，接种物-I导致的定殖率高于接种物-II，但定殖主要在盲肠中。接种物-II对B组的影响与水对C组的影响相似，仅显示对定殖的轻微影响。微阵列分析显示，每组在回肠中呈现不同的基因表达模式。在A组中，控制离子转运、细胞周期和染色体维持功能的基因变化最明显，表明接种物影响了基因表达。我们的研究结果表明，最初暴露于不同的细菌群落可能导致肠道中不同微生物群和基因表达的发展。通过在早期喂养适当的细菌组成来操纵肠道微生物群是可能的。
The transition from a sterile gut environment to the development of microbiota in the newborns is not fully understood. The objective of this study was to investigate the impact of exposure to bacterial communities on the development of gut microbiota in the newly hatched chicken. A total of 90 as-hatched chicks were divided into three groups. Groups A and B were treated with inocula of the cecal origin, whereas group C was fed with sterile water. The major bacteria in Inoculum-I to treat group A includedBacteroides(20.7%),Lachnospiraceae(17.2%) and unclassifiedRuminococcaceae(16.1%), whereas group B was introduced with Inoculum-II composed ofPrevotella(37.9%),Acidaminococcus(16.1%) andDorea(12.6%). Analyses of the ileal and cecal contents over a period of 15 days showed that Inoculum-I resulted in a higher rate of colonization than Inoculum-II, but the colonization was predominantly in the cecum. The influence of Inoculum-II on group B was similar to that of water on group C, showing only a marginal effect on colonization. Microarray analysis showed that each group presented a distinct pattern of gene expression in the ileum. In group A, the most obvious changes were noted in genes controlling the function of ion transport, cell cycle and chromosome maintenance, suggesting that the inocula influenced gene expression. Our findings indicate that initial exposure to different bacterial communities could lead to the development of distinct microbiota and gene expression in the gut. It is possible to manipulate the gut microbiota by feeding to a proper bacterial composition at an early age.