D and 18O enrichment measurements in biological fluids in a continuous-flow elemental analyser with an isotope-ratio mass spectrometer using two configurations
D and 18O enrichment measurements in biological fluids in a continuous-flow elemental analyser with an isotope-ratio mass spectrometer using two configurations
复制标题
DOI：
10.1002/jms.1093
复制
发表时间：
2006-09-01
影响因子：
2.3
通讯作者：
Ritz, P.
中科院分区：
文献类型：
--
作者：
Ripoche, N.;Ferchaud-Roucher, V.;Ritz, P.
In doubly labelled water studies, biological sample enrichments are mainly measured using off-line techniques (equilibration followed by dual-inlet introduction) or high-temperature elemental analysis (HT-EA), coupled with an isotope-ratio mass spectrometer (IRMS). Here another continuous-flow method, (CF-EA/IRMS), initially dedicated to water, is tested for plasma and urine analyses.The elemental analyser configuration is adapted for each stable isotope: chromium tube for deuterium reduction and glassy carbon reactor for 180 pyrolysis. Before on-line conversion of water into gas, each matrix is submitted to a short and easy treatment, which is the same for the analysis of the two isotopes.血浆通过离心过滤器。 Urine is cleaned with black carbon and filtered (0.45 mu m diameter).Tested between 150 and 300 ppm in these fluids, the D/H ratio response is linear with good repeatability (SD < 0.2 ppm) and reproducibility (SD < 0.5 ppm).对于 180/160 比率（从 2000 到 2200 ppm），可以获得相同的重复性，但日间精度低于 1.4 ppm。通过与经典双进样方法进行比较，验证了生物样品的准确性：O-18 分析可提供更准确的结果。数据表明，可以在 CF-EA/IRMS 中成功分析富集的生理液体。版权所有 (c) 2006 John Wiley & Sons, Ltd.
In doubly labelled water studies, biological sample enrichments are mainly measured using off-line techniques (equilibration followed by dual-inlet introduction) or high-temperature elemental analysis (HT-EA), coupled with an isotope-ratio mass spectrometer (IRMS). Here another continuous-flow method, (CF-EA/IRMS), initially dedicated to water, is tested for plasma and urine analyses.The elemental analyser configuration is adapted for each stable isotope: chromium tube for deuterium reduction and glassy carbon reactor for 180 pyrolysis. Before on-line conversion of water into gas, each matrix is submitted to a short and easy treatment, which is the same for the analysis of the two isotopes. Plasma is passed through centrifugal filters. Urine is cleaned with black carbon and filtered (0.45 mu m diameter).Tested between 150 and 300 ppm in these fluids, the D/H ratio response is linear with good repeatability (SD < 0.2 ppm) and reproducibility (SD < 0.5 ppm). For 180/160 ratios (from 2000 to 2200 ppm), the same repeatability is obtained with a between-day precision lower than 1.4 ppm. The accuracy on biological samples is validated by comparison to classical dual-inlet methods: O-18 analyses give more accurate results. The data show that enriched physiological fluids can be successfully analysed in CF-EA/IRMS. Copyright (c) 2006 John Wiley & Sons, Ltd.