Involvement of the abscisic acid catabolic gene CYP707A2 in the glucose-induced delay in seed germination and post-germination growth of Arabidopsis.
Involvement of the abscisic acid catabolic gene CYP707A2 in the glucose-induced delay in seed germination and post-germination growth of Arabidopsis.
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DOI：
10.1111/j.1399-3054.2011.01510.x
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发表时间：
2011-12
影响因子：
6.4
通讯作者：
Guohui Zhu;Ying-Gao Liu;Nenghui Ye;Rui Liu;Jianhua Zhang
中科院分区：
文献类型：
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作者：
Guohui Zhu;Ying-Gao Liu;Nenghui Ye;Rui Liu;Jianhua Zhang
早期的研究表明，糖作为信号分子在种子萌发的调控中起着关键作用。阿坝生物合成上调被认为是葡萄糖诱导延迟种子萌发的可能机制之一。在这项研究中，阿坝catalysts在葡萄糖诱导的抑制作用的作用进行了研究。以拟南芥种子为材料，研究表明烯唑醇对阿坝催化剂的抑制加重了葡萄糖诱导的种子萌发延迟。外源葡萄糖处理显著降低了拟南芥阿坝催化剂中编码阿坝8 '-羟化酶的关键基因CYP 707 A2的转录和蛋白表达。转基因实验证实，CYP 707 A2基因的过表达减轻了葡萄糖诱导的抑制作用，而CYP 707 A2突变体种子在吸胀过程中表现出对葡萄糖的超敏反应。外源葡萄糖也抑制了拟南芥幼苗的早期发育。与野生型幼苗相比，CYP 707 A2过表达幼苗表现出较低的阿坝水平，似乎对外源葡萄糖不太敏感。总之，葡萄糖诱导的种子萌发和幼苗发育延迟与通过抑制CYP 707 A2表达而抑制阿坝catalysts直接相关。
Earlier studies showed that sugars as signaling molecules play pivotal roles in the regulation of seed germination. ABA biosynthesis upregulation is suggested as one of the possible mechanisms mediating the glucose-induced delay in seed germination. In this study, the role of ABA catabolism in glucose-induced inhibition was investigated. Using Arabidopsis thaliana seeds, the results show that the repression of ABA catabolism by diniconazole aggravated the glucose-induced delay in seed germination. The transcript and protein profiles of CYP707A2, a key gene encoding ABA 8'-hydroxylase in ABA catabolism in Arabidopsis, were significantly decreased by exogenous glucose treatment. Transgenic experiments confirmed that the over-expression of the CYP707A2 gene alleviated the glucose-induced inhibition effect, whereas the cyp707a2 mutant seeds displayed a hypersensitivity to glucose during imbibition. Exogenous glucose also arrested the early seedling development of Arabidopsis. The CYP707A2 over-expression seedlings exhibited lower ABA levels and seemed less sensitive to exogenous glucose compared with wild type seedlings. In summary, the glucose-induced delay in seed germination and seedling development is directly related to the suppression of ABA catabolism through the repression of the CYP707A2 expression.