Air-drying of cells enables visualization of antiparallel microtubule overlaps in the spindle midzone.
Air-drying of cells enables visualization of antiparallel microtubule overlaps in the spindle midzone.
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DOI：
10.1016/j.mex.2018.04.011
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发表时间：
2018
期刊：
影响因子：
1.9
通讯作者：
Nakayama Y
中科院分区：
文献类型：
--
作者：
Ifuji A;Kuga T;Nakayama Y
免疫荧光染色被广泛用于检测各种类型的细胞事件。然而，即使一种抗体在免疫印迹中可以检测到它的表位，当用于免疫荧光染色时，它有时也无法检测到它的表位。一个例子是在后期和末期纺锤体中区重叠的反平行微管，它作为分裂沟规范的信号支架。人们认为，由于微管重叠的高密度结构，它不能通过免疫荧光染色显示(Ifuji等人，2017年)。在这里，我们展示了一种简单的方法来显示纺锤体中后期和末期的反平行微管重叠。固定前风干细胞可以显示纺锤体中后期和末期的反平行微管重叠，这是常规方法无法看到的。简单的方法，只需最少的设备使用。在这种方法中可以使用常用的抗微管蛋白抗体。
Immunofluorescence staining is used extensively to examine various types of cellular events. However, even when an antibody can detect its epitopes in western blotting, it sometimes fails to detect its epitopes when used for immunofluorescence staining. One example is the antiparallel microtubule overlaps in the anaphase and telophase spindle midzone, which functions as a signaling scaffold for cleavage furrow specification. It has been believed that it cannot be visualized by immunofluorescence staining due to the highly dense structure of microtubule overlaps (Ifuji et al., 2017). Here, we show a simple method for visualization of antiparallel microtubule overlaps in the anaphase and telophase spindle midzone. Air-drying cells before fixation enables visualization of antiparallel microtubule overlaps in the anaphase and telophase spindle midzone, which cannot be visualized by the conventional method. Simple method that requires minimal usage of equipment. Commonly used anti-tubulin antibodies can be used in this method.