The long and short of non-coding RNAs during post-natal growth and differentiation of skeletal muscles: Focus on lncRNA and miRNAs
The long and short of non-coding RNAs during post-natal growth and differentiation of skeletal muscles: Focus on lncRNA and miRNAs
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DOI：
10.1016/j.diff.2016.05.003
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发表时间：
2016-12-01
期刊：
影响因子：
2.9
通讯作者：
Grounds, Miranda D.
中科院分区：
文献类型：
--
作者：
Butchart, Lauren C.;Fox, Archa;Grounds, Miranda D.
骨骼肌的出生后生长是一个动态过程，涉及成肌细胞的增殖和融合，以及肌纤维的伸长（肌核增生），直到小鼠出生后 3 周，持续分化，肌纤维尺寸进一步增加，主要通过肥大，直到大约 12 周龄。控制这些事件的 mRNA 的表达已得到很好的描述，但对非编码 RNA (ncRNA) 的体内作用知之甚少，包括 microRNA (miRNA) 和研究较少的长非编码 RNA (lncRNA)。我们分析了正常雄性 C57Bl/6J 小鼠出生后 2 天以及 2、4、6 和 12 周肌肉中各种 IncRNA（包括 Neat1、Malat1、Sra、Meg3、LncMyoD 和 lint-MD1）、miRNA 和 mRNA 的表达模式。将这些出生后模式与组织培养中经典 C2C12 肌发生和分化过程中这些 RNA 的表达进行比较。对出生后骨骼肌生长过程中 RNA 的概述为这个经常被忽视的生长时期的 ncRNA 提供了新的关注点，它在人类和牲畜的正常肌肉生长以及儿童肌肉疾病方面具有许多潜在的应用。 (C) 2016 年国际分化学会。由 Elsevier B.V. 出版。保留所有权利。
Post-natal growth of skeletal muscle is a dynamic process involving proliferation and fusion of myoblasts with elongating myofibres (hyperplasia of myonuclei) until 3 weeks post-natally in mice, with ongoing differentiation and further increases in myofibre size mostly by hypertrophy until about 12 weeks of age. The expression of mRNAs that control these events are well described, but little is known about the in vivo roles of non-coding RNAs (ncRNAs), including both microRNAs (miRNAs) and the lesser-studied long non-coding RNAs (lncRNAs). We analysed expression patterns for a broad range of IncRNAs (including Neat1, Malat1, Sra, Meg3, LncMyoD and lint-MD1), miRNAs and mRNAs in muscles of normal male C57Bl/6J mice at 2 days and 2, 4, 6 and 12 weeks after birth. These post-natal patterns were compared with expression of these RNAs during classic C2C12 myogenesis and differentiation in tissue culture. This overview of RNAs during post-natal skeletal muscle growth provides a novel focus on ncRNAs during this often overlooked growth period, with many potential applications to normal muscle growth in humans and livestock, and to childhood muscle disorders. (C) 2016 International Society of Differentiation. Published by Elsevier B.V. All rights reserved.