Anti-inflammatory and Mineralization Effects of ProRoot MTA and Endocem MTA in Studies of Human and Rat Dental Pulps In Vitro and In Vivo
Anti-inflammatory and Mineralization Effects of ProRoot MTA and Endocem MTA in Studies of Human and Rat Dental Pulps In Vitro and In Vivo
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DOI：
10.1016/j.joen.2018.07.012
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发表时间：
2018-10-01
影响因子：
4.2
通讯作者：
Hwang, Yun-Chan
中科院分区：
文献类型：
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作者：
Kim, Do-Hee;Fang, Ji-Hyun;Hwang, Yun-Chan
引言：很少有研究报道直接盖髓在炎症牙髓条件。本研究的目的是探讨在炎症条件下，使用不同盖髓材料进行直接盖髓过程中牙髓的体外和体内反应。研究方法：用脂多糖（LPS）处理人牙髓细胞，并用Dycal（Dentsply Caulk，米尔福德，DE）、ProRoot MTA（Dentsply Maillefer，Ballaigues，瑞士）和Endocem MTA（Maruchi，元州，韩国）培养。实时荧光定量聚合酶链反应检测IL-10、IL-6、牙本质基质蛋白1和牙本质涎磷蛋白的表达。将Sprague-Dawley大鼠的上颌磨牙暴露2天。暴露的牙髓用Dycal、Pro Root MTA和Endocem MTA覆盖，并用树脂改性玻璃离子密封，然后进行组织学和免疫组织化学分析。结果：LPS可使IL-1#、IL-6表达增加，Dycal、ProRoot MTA、Endocem MTA可使IL-1#、IL-6表达减少。牙本质基质蛋白1和牙本质唾液磷蛋白水平在LPS作用下降低，而在盖髓材料作用后升高。在体内研究中，在第1周时，Dycal相关的炎症高于ProRoot MTA和Endocem MTA相关的炎症，但两者之间无任何显著差异。在第4周，与第1周相比，所有3种材料的炎症减少，矿化增加。在第1周，IL-6免疫反应性强表达。Dycal的免疫反应性强于ProRoot MTA和Endocem MTA。然而，在第4周，所有组的免疫反应性均降低。结论：成功的直接盖髓需要更有效的盖髓材料。
Introduction: Few studies have reported direct pulp capping in inflamed pulp conditions. The purpose of this study was to investigate the in vitro and in vivo responses of dental pulp during direct pulp capping using various pulp capping materials in inflamed conditions. Methods: Human dental pulp cells were treated with lipopolysaccharide (LPS) and cultured with Dycal (Dentsply Caulk, Milford, DE), ProRoot MTA (Dentsply Maillefer, Ballaigues, Switzerland), and Endocem MTA (Maruchi, Wonju, South Korea). The expressions of interleukin (IL)-10, IL-6, dentin matrix protein 1, and dentin sialophosphoprotein were analyzed through real-time polymerase chain reaction. The maxillary molars of Sprague-Dawley rats were exposed for 2 days. The exposed pulps were capped with Dycal, Pro Root MTA, and Endocem MTA and sealed with resin modified glass ionomer followed by histologic and immunohistochemical analyses. Results: The expression of IL-1# and IL-6 was increased with LPS and decreased by Dycal, ProRoot MTA, and Endocem MTA. Dentin matrix protein 1 and dentin sialophosphoprotein levels were decreased with LPS and increased after treatment with pulp capping materials.ln the in vivo study, inflammation associated with Dycal was higher than that associated with ProRoot MTA and Endocem MTA at week 1, without any significant difference between the 2. At 4 weeks, inflammation was decreased, and mineralization was increased compared with week 1 in all 3 of the materials. At week 1, IL-6 immunoreactivity was strongly expressed. Dycal exhibited stronger immunoreactivity than ProRoot MTA and Endocem MTA. However, the immunoreactivity was decreased in all groups at week 4. Conclusions: Successful direct pulp capping requires more effective pulp capping materials for the treatment of inflamed pulps.