Histopathologic features of melanocytic tumors in Xiphophorus melanoma receptor kinase (xmrk)-transgenic medaka (Oryzias latipes)
Histopathologic features of melanocytic tumors in Xiphophorus melanoma receptor kinase (xmrk)-transgenic medaka (Oryzias latipes)
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剑尾鱼黑色素瘤受体激酶 (xmrk) 转基因青鳉 (Oryzias latipes) 黑色素细胞肿瘤的组织病理学特征
DOI：
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发表时间：
2019
影响因子：
1.2
通讯作者：
K. Naruse
中科院分区：
文献类型：
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作者：
A. Sugiyama;M. Schartl;K. Naruse
黑色素细胞肿瘤剑尾鱼黑色素瘤受体激酶（xmrk）转基因卡比奥和HB11A青鳉株进行了组织病理学检查，在孵化后7个月。两个品系的青鳉均发生了黑素细胞瘤，有效率为100%。在这两种菌株中，含有胞浆内黑色素颗粒的肿瘤细胞表现出显着的侵袭性生长模式。此外，上皮样瘤细胞呈实性巢状排列，梭形瘤细胞呈交错的溪流和束状排列。在两种青鳉品系的肿瘤病变中均观察到核异型性、核不全、细胞多态性以及含有多个核或单个核的间变性巨细胞的出现。然而，无论是菌株表现出有丝分裂的数字或侵入血管的肿瘤细胞。根据这些组织病理学结果，肿瘤被诊断为恶性黑色素瘤。这是第一次报告的xmrk转基因青鳉中恶性黑色素瘤的详细组织形态学特征。
Melanocytic tumors in Xiphophorus melanoma receptor kinase (xmrk)-transgenic Carbio and HB11A strains of medaka were examined histopathologically at 7 months post-hatching. Medaka of both strains developed melanocytic tumors with a penetrance of 100%. In both strains, neoplastic cells containing intracytoplasmic melanin pigment granules showed significant invasive growth patterns. In addition, epithelioid neoplastic cells were arranged in solid nests, and spindle neoplastic cells were arranged in interlacing streams and bundles. Nuclear atypia, anisokaryosis, cellular pleomorphism, and the appearance of anaplastic giant cells containing multiple nuclei or a single nucleus were observed in neoplastic lesions in both medaka strains. However, neither strain exhibited mitotic figures or invasion of blood vessels by neoplastic cells. Based on these histopathologic findings, the tumors were diagnosed as malignant melanoma. This is the first report of detailed histomorphologic characteristics of malignant melanoma in xmrk-transgenic medaka.