RAPID VIABILITY LOSS ON EXPOSURE TO AIR IN A SUPEROXIDE DISMUTASE-DEFICIENT MUTANT OF PORPHYROMONAS-GINGIVALIS
RAPID VIABILITY LOSS ON EXPOSURE TO AIR IN A SUPEROXIDE DISMUTASE-DEFICIENT MUTANT OF PORPHYROMONAS-GINGIVALIS
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DOI：
10.1128/jb.176.7.1939-1943.1994
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发表时间：
1994-04-01
影响因子：
3.2
通讯作者：
NAKAYAMA, K
中科院分区：
文献类型：
--
作者：
NAKAYAMA, K
牙龈卟啉单胞菌是一种专性厌氧菌，具有较高的耐氧性和超氧化物歧化酶(SOD)，可通过暴露在空气中诱导产生。为了阐明超氧化物歧化酶在该生物体中的作用，利用含有突变的超氧化物歧化酶的自杀质粒，以基因导向的方式破坏了牙龈假单胞菌染色体上编码超氧化物歧化酶(Sod)的基因。获得的一株超氧化物歧化酶突变体的粗提物中没有显示出超氧化物歧化酶活性，暴露于空气后立即表现出迅速的活力丧失，而野生型亲本在有氧条件下至少5h的活力没有下降。这些结果清楚地表明，超氧化物歧化酶在牙龈假单胞菌的耐氧性中是必不可少的。
Porphyromonas gingivalis, an obligate anaerobe, exhibits a relatively high degree of aerotolerance and possesses superoxide dismutase (SOD) which is induced by exposure to air. To clarify roles for SOD in this organism, the gene encoding SOD (sod) on the P. gingivalis chromosome was disrupted in a gene-directed way by use of a suicide plasmid containing a mutated sod. A sod mutant thus obtained showed no SOD activity in crude extracts and exhibited a rapid viability loss immediately after exposure to air, whereas the wild-type parent showed no decrease in viability for at least 5 h under aerobic conditions. These results clearly indicate that SOD is essential for aerotolerance in P. gingivalis.