ELECTRIC-STIMULATION OF PROTEIN AND DNA-SYNTHESIS IN HUMAN-FIBROBLASTS
ELECTRIC-STIMULATION OF PROTEIN AND DNA-SYNTHESIS IN HUMAN-FIBROBLASTS
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DOI：
10.1096/fasebj.1.5.3678699
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发表时间：
1987-11-01
期刊：
影响因子：
4.8
通讯作者：
BOURGUIGNON, LYW
中科院分区：
文献类型：
--
作者：
BOURGUIGNON, GJ;BOURGUIGNON, LYW
采用人成纤维细胞培养物作为模型系统，以快速检查涉及使用高压脉冲电刺激（HVPGS）提高软组织损伤愈合率的几个潜在重要变量。成纤维细胞在Millipore过滤器上生长，并在填充有生长培养基的矩形塑料室中暴露于各种电压和脉冲速率的HVPGS 20分钟。将附着有细胞的过滤器放置在室的中心或靠近正极或负极。分别用放射性标记的前体[3 H]脯氨酸和[3 H]胸苷刺激后监测蛋白质合成和DNA合成。结果表明：HVPGS电压和脉冲频率的特定组合可显著提高细胞蛋白质和DNA的合成速率，在50和75 V，脉冲频率为100 pulses/s，细胞位于负电极附近时，蛋白质和DNA的合成速率最大;和3）暴露于大于250 V的HVPGS强度（在腔室内的所有脉冲速率和位置）对蛋白质和DNA合成都是抑制性的。鉴于使用HVPGS治疗皮肤溃疡的初步临床研究中获得的结果，似乎需要相似的电压、脉冲频率和相对电极位置来最大限度地加速人体皮肤伤口愈合。
Human fibroblast cell cultures were employed as a model system to rapidly examine several potentially important variables involved in the use of high-voltage, pulsed galvanic stimulation (HVPGS) on increased the healing rate of soft tissue injuries. Fibroblasts were grown on Millipore filters and exposed to HVPGS of various voltages and pulse rates for 20 min in a rectangular, plastic chamber filled with growth medium. Filters with attached cells were placed either in the center of the chamber or close to the positive or negative electrode. Protein synthesis and DNA synthesis were monitored after stimulation using the radioactively labeled precursors, [3H]proline and [3H]thymidine, respectively. The major results obtained in this study are as follows: 1) the rates of both protein and DNA synthesis can be significantly increased by specific combinations of HVPGS voltage and pulse rate; 2) maximum stimulation of protein and DNA synthesis was obtained at 50 and 75 V, respectively, with a pulse rate of 100 pulses/s and the cells located near the negative electrode; and 3) exposure to HVPGS intensities greater than 250 V (at all pulse rates and locations within the chamber) is inhibitory for both protein and DNA synthesis. In view of the results obtained in preliminary clinical studies on the use of HVPGS for the treatment of dermal ulcers, it appears that similar voltages, pulse rates, and relative electrode location may be required for maximum acceleration of human skin wound healing.