STRUCTURAL-ANALYSIS OF COMPLEMENTARY-DNA AND AMINO-ACID SEQUENCES OF HUMAN AND RAT ANDROGEN RECEPTORS
STRUCTURAL-ANALYSIS OF COMPLEMENTARY-DNA AND AMINO-ACID SEQUENCES OF HUMAN AND RAT ANDROGEN RECEPTORS
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DOI：
10.1073/pnas.85.19.7211
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发表时间：
1988-10-01
影响因子：
11.1
通讯作者：
LIAO, SS
中科院分区：
文献类型：
--
作者：
CHANG, CS;KOKONTIS, J;LIAO, SS
对人和大鼠雄激素受体 (AR) cDNA 的结构分析表明，AR 的氨基末端区域富含寡聚和聚（氨基酸）基序，如某些同源异型基因中一样。人类 AR 具有一长段重复甘氨酸，而大鼠 AR 具有一长段谷氨酰胺。 AR 与类固醇结合域内的糖皮质激素、孕激素和盐皮质激素受体之间存在相当大的序列相似性。富含半胱氨酸的 DNA 结合域非常保守。从 AR cDNA 转录的 mRNA 的翻译产生了 94 kDa 和 76 kDa 的蛋白质以及与 DNA 结合并对雄激素具有高亲和力的较小形式。这些大鼠或人类 AR 被天然 AR 的人类自身抗体识别。使用AR cDNA作为探针的分子杂交研究表明，腹侧前列腺和其他男性附属器官富含AR mRNA，并且靶器官中AR mRNA的产生可能受到雄激素的自动调节。
Structural analysis of cDNAs for human and rat androgen receptors (ARs) indicates that the amino-terminal regions of ARs are rich in oligo- and poly (amino acid) motifs as in some homeotic genes. The human AR has a long stretch of repeated glycines, whereas rat AR has a long stretch of glutamines. There is a considerable sequence similarity among ARs and the receptors for glucocorticoids, progestins, and mineralocorticords within the steroid-binding domains. The cysteine-rich DNA-binding domains are well conserved. Translation of mRNA transcribed from AR cDNAs yielded 94- and 76-kDa proteins and smaller forms that bind to DNA and have high affinity toward androgens. These rat or human ARs were recognized by human autoantibodies to natural ARs. Molecular hybridization studies, using AR cDNAs as probes, indicated that the ventral prostate and other male accessory organs are rich in AR mRNA and that the production of AR mRNA in the target organs may be autoregulated by androgens.