Involvement of superoxide in the interaction of 2,3-dichloro-1,4-naphthoquinone with mitochondrial membranes.
Involvement of superoxide in the interaction of 2,3-dichloro-1,4-naphthoquinone with mitochondrial membranes.
复制标题
超氧化物参与 2,3-二氯-1,4-萘醌与线粒体膜的相互作用。
DOI：
10.1016/0003-9861(82)90483-0
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发表时间：
1982
影响因子：
3.9
通讯作者：
Ronald S. Pardini
中科院分区：
文献类型：
--
作者：
C. Pritsos;David E. Jensen;D. Pisani;Ronald S. Pardini
2，3-二氯-1，4-萘醌（CNQ）在呼吸链复合物I和II的不同位点上抑制牛心线粒体呼吸。琥珀氧化酶和NADH氧化酶系统的I50值分别为18和9 nmol/mg蛋白。CNQ与大鼠肝线粒体的相互作用引起由氰化物敏感组分和氰化物不敏感组分组成的呼吸爆发。CNQ呼吸爆发的氰化物敏感部分被鉴定为氧化磷酸化的解偶联。氰化物不敏感的部分被证明涉及O2-，H2 O2的产生，并导致随后的脂质过氧化。CNQ介导的O2-和H2 O2生成的还原当量来源是呼吸底物。此外，CNQ促进线粒体的剂量依赖性大幅度肿胀，这是渗透性的，阳离子非特异性的，非能量连接的，和氧依赖性的。在悬浮缓冲液中包含半胱氨酸防止了CNQ诱导的线粒体肿胀。O2-、H2 O2和过氧化作用在CNQ诱导的线粒体肿胀中的作用进行了讨论。
2,3-Dichloro-1,4-naphthoquinone (CNQ) was shown to inhibit beef heart mitochondrial respiration at separate and distinct sites in Complexes I and II of the respiratory chain. The I50values were 18 and 9 nmol/mg protein for the succinoxidase and NADH-oxidase enzyme systems, respectively. The interaction of CNQ with rat liver mitochondria causes a respiratory burst which is comprised of cyanide-sensitive and -insensitive components. The cyanide-sensitive portion of the CNQ respiratory burst was identified as uncoupling of oxidative phosphorylation. The cyanide-insensitive portion was shown to involve the generation of O2−, H2O2, and result in subsequent lipid peroxidation. The source of reducing equivalents for the CNQ-mediated generation of O2−and H2O2was shown to be respiratory substrate. Furthermore, CNQ promoted dose-dependent large-amplitude swelling of mitochondria that was osmotic in nature, cation nonspecific, non-energy linked, and oxygen dependent. The inclusion of cysteine in the suspension buffer prevented the CNQ-induced mitochondrial swelling. The role of O2−, H2O2, and peroxidation in the mitochondrial swelling induced by CNQ was discussed.
2,3-双（氯甲基）-1,4-萘醌诱导线粒体肿胀的表征。
影响因子：
5.1
作者：
Pardini,RS;Tilka,MA;Pritsos,CA;Lin,AJ;Sartorelli,AC
通讯作者：
Sartorelli,AC