Differences of two polychaete species reflected in enzyme activities
Differences of two polychaete species reflected in enzyme activities
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DOI：
10.1007/s00227-011-1641-7
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发表时间：
2011-02
期刊：
影响因子：
2.4
通讯作者：
K. Ito;M. Nozaki;Takashi Ohta;C. Miura;Y. Tozawa;T. Miura
中科院分区：
文献类型：
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作者：
K. Ito;M. Nozaki;Takashi Ohta;C. Miura;Y. Tozawa;T. Miura
多毛类构成了河口和沿海环境中大多数底栖大型无脊椎动物。对两种多毛类植物Capitellasp对有机质的利用进行了研究。I和Perinereis nuna brevicirris，生活在不同的沿海栖息地。Capitellasp的蛋白酶活性。I(89.7gμg，mg−1)约为OFP的10倍。μg mg−1)，纤维素酶(β-1，4-葡聚糖酶)活性较高。Nuna brevicirris(3.2gμg/mg−1)，而Capitellasp几乎没有检测到。I.我们从Capitellasp中分离到了蛋白水解酶mRNA的cDNA克隆。I和纤维素酶mRNA的表达。短毛修女。Capitellasp具有较高的蛋白酶活性。我让它在养鱼场下面的沉积物中存活下来，在那里它能降解有机物。相反，河口的纤维素酶活性较高。短修女使其能够降解来自陆地地区的有机物。
Polychaetes constitute most of the benthic macroinvertebrates in estuarine and coastal environments. We investigated the utilization of organic matter in two polychaete species,Capitellasp. I andPerinereis nuntia brevicirris, living in different coastal habitats. The protease activity ofCapitellasp. I (89.7 μg mg−1) was about 10 times that ofP. nuntia brevicirris(8.0 μg mg−1).High cellulase (endo-β-1,4-glucanase) activity was detected inP. nuntia brevicirris(3.2 μg mg−1), whereas scarcely any was detected inCapitellasp. I. We isolated cDNA clones of protease mRNA fromCapitellasp. I and of cellulase mRNA fromP. nuntia brevicirris. The high protease activity ofCapitellasp. I enabled it to survive in the sediment under a fish farm, where it degrades organic matter. In contrast, the high cellulase activity of the estuary-dwellingP. nuntia brevicirrisallowed it to degrade organic matter originating from terrestrial areas.