Artificial Light at Night (ALAN), an alarm to ovarian physiology: A study of possible chronodisruption on zebrafish (Danio rerio)
Artificial Light at Night (ALAN), an alarm to ovarian physiology: A study of possible chronodisruption on zebrafish (Danio rerio)
复制标题
DOI：
10.1016/j.scitotenv.2018.02.101
复制
发表时间：
2018-07-01
影响因子：
9.8
通讯作者：
Chattoraj, Asamanja
中科院分区：
文献类型：
--
作者：
Khan, Zeeshan Ahmad;Labala, Rajendra Kumar;Chattoraj, Asamanja
ALAN引起了研究人员和环保主义者的注意，因为越来越多的证据表明它具有生物体生理学的“去寄生化”能力。光周期和昼夜节律周期是影响包括鱼类在内的几种动物生殖生物学的关键参数。目前的研究是第一次证明卵巢肿瘤的发展与光的影响，斑马鱼（Danio rerio），一个很好的模型，昼夜节律相关的研究。三种实验条件（连续光照一周、LLW、一个月、LLM和一年、LLY）的结果显示，时钟相关基因（Clock 1a、Bmal 1a、Per 2和Cry 2a）明显失同步。有趣的是，通过ELISA在这些条件下在全脑、视网膜、卵巢和血清中发现节律性丧失和低浓度的褪黑激素。卵巢样本的RNA-Seq数据揭示了Mid 2、Tfg、Pim 1、Pim 2、Tradd、Tmem 101、Nfkbib基因的上调，并最终增加了NF-κ B的表达，NF-κ B是肿瘤发生的细胞Transformer，通过蛋白质印迹证实。TNF α、炎性细胞因子和NF-κ B B激活剂的出现也增加。组织学证实在暴露一年的卵巢样本中形成了卵泡膜细胞瘤和颗粒细胞瘤。全转录组数据分析显示，卵巢肿瘤中有1791个基因显著上调。在这些基因中，大卫功能注释工具鉴定了438个基因，直接与其他生理疾病相关。这项研究证明了由ALAN在斑马鱼中诱导的卵巢肿瘤。(C)2018 Elsevier B. V.版权所有。
The ALAN is drawing the attention of researchers and environmentalists for its ever-increasing evidence on its capacity of "desynchronization" of organismal physiology. Photoperiod and circadian cycles are critical parameters to influence the biology of reproduction in several animals, including fish. The present study is the first proof of the development of an ovarian tumour with the effect of light in zebrafish (Danio rerio), an excellent model for circadian-related studies. Results of three experimental conditions, continuous light for one week, LLW, one month, LLM, and for one year, LLY revealed a dear desynchronization of clock associated genes (Clock1a, Bmal1a, Per2, and Cry2a). Interestingly, loss of rhythmicity and low concentration of melatonin found in these conditions in whole brain, retina, ovary, and serum through ELISA. RNA-Seq data of ovarian samples revealed the upregulation of Mid2, Tfg, Irak1, Pim2, Tradd, Tmem101, Nfkbib genes and ultimately increase the expression of NF-kappa B, a cellular transformer for tumourigenesis, confirmed by the western blot. The appearance of TNF alpha, inflammatory cytokines and activator of NF-kappa B also increased. Histology approved the formation of thecoma and granulosa cell tumour in the one year exposed ovarian sample. The whole transcriptome data analysis revealed 1791 significantly upregulated genes in an ovarian tumour. Among these genes, DAVID functional annotation tool identified 438 genes, directly linked to other physiological disorders. This study evidenced of an ovarian tumour induced by ALAN in zebrafish. (C) 2018 Elsevier B.V. All rights reserved.