Mouse models of hepatocyte biology - Known unknowns.
Mouse models of hepatocyte biology - Known unknowns.
复制标题
肝细胞生物学的小鼠模型 - 已知的未知数。
DOI：
10.1016/j.jhep.2023.02.002
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发表时间：
2023
影响因子：
25.7
通讯作者：
Hoare M
中科院分区：
文献类型：
--
作者：
Hoare M
小鼠模型对于许多重要的机制发现至关重要，这些发现支撑了我们对肝脏疾病病理生理学的理解。操纵基因和途径的能力使我们能够建立因果关系，使它们成为肝脏相关研究中的宝贵工具。近年来，随着CRISPR/Cas9的使用，新型遗传模型的开发加速，允许更快，更准确的基因组编辑。然而，已经变得明显的是，基因组修饰不是简单的确定性的，并且只有通过仔细研究和验证模型才能识别出超出预期靶位点的潜在非预期后果。在本期Journal ofHepatology的一项有趣的新工作中，May等人[1]对这种模型进行了详细的检查，虽然精细的细节对肝脏稳态和再生领域的人很重要，但他们的发现对所有在研究中使用小鼠模型的人具有更广泛的意义。
Mouse models have been fundamental to many significant mechanistic findings that underpin our understanding of the pathophysiology of liver disease. The ability to manipulate genes and pathways allowing us to establish causation, makes them an invaluable tool in liver-related research. Development of novel genetic models has accelerated over recent years with the use of CRISPR/Cas9, permitting faster and more accurate genome editing. However, it has become apparent that genomic modification is not simply deterministic and that potential unintended consequences beyond the intended target locus will only be identified with careful study and validation of the model. In a fascinating new work in this issue of Journal ofHepatology, May et al [1] have performed a detailed examination of such a model and whilst the fine details are important to those in the field of liver homeostasis and regeneration, their findings have broader implications to all who use mouse models in their research.