Neurobehavioral development of two mouse lines commonly used in transgenic studies
Neurobehavioral development of two mouse lines commonly used in transgenic studies
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DOI：
10.1016/s0091-3057(02)00792-x
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发表时间：
2002-08-01
影响因子：
3.6
通讯作者：
Estivill, X
中科院分区：
文献类型：
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作者：
Dierssen, M;Fotaki, V;Estivill, X
本研究旨在确定源自两个 F I 杂交小鼠品系（129 x C57BL/6 和 C57BL/6 x SJL）的两个 F2 系之间神经发育特征的差异。给定品系的选择是基于这些小鼠在转基因研究中的频繁使用。对于神经发育表型分析，我们采用了由 23 项躯体测量、感觉和运动测试组成的测试组。在不同的功能域中建立了菌株之间的显着差异。在 F1 C57BL/6 x 129 杂交衍生的 F2 小鼠中观察到发育标志出现的一些特定延迟，而它们比 C57BL/6 x SJL 衍生小鼠更早获得早期发育功能，例如翻正反射。 C57BL/6 x 129 F2 后代自发性机能减退，其较差的运动表现通过负趋地性测试中的低表现得到证实。然而，两个 F2 品系在归巢测试中的良好表现表明，一般精神运动发育没有差异。两种品系都对所使用的处理程序敏感，与未处理的对照小鼠相比，在归巢测试中观察到的反应出现类似的变化。总之，我们的工作强调了转基因实验遗传背景的重要性，以及在转基因小鼠行为筛选的第一阶段获得充分信息的完善测试方案的必要性。 (C) 2002 Elsevier Science Inc. 保留所有权利。
The present study was aimed at establishing the differences in the neurodevelopmental profile between two F2 lines derived from two F I hybrid mouse strains (129 x C57BL/6 and C57BL/6 x SJL). The choice of the given strains was based on the frequent use of these mice in transgenic research. For the neurodevelopment phenotyping, we employed a test battery consisting of 23 somatometric, sensorial and motor tests. Significant variations between the strains were established in different functional domains. Some specific delays in the appearance of developmental landmarks were observed in F2 mice derived from crosses of F1 C57BL/6 x 129, whereas they acquired early developmental functions, such as the righting reflex, sooner than C57BL/6 x SJL-derived mice. C57BL/6 x 129 F2 offspring were spontaneously hypoactive, and their poorer motor performance was confirmed by low performance in the negative geotaxis test. However, there were no differences in the general psychomotor development as shown by the good performance in the homing test in both F2 lines. Both strains were susceptible to the handling procedures used, presenting a similar alteration in the response observed in the homing test as compared to nonhandled control mice. In conclusion, our work highlights the importance of the genetic background for transgenesis experiments and also the need for well-established testing protocols to obtain sufficient information at the first stage of behavioral screening of genetically modified mice. (C) 2002 Elsevier Science Inc. All rights reserved.