In vivo integrated flow image cytometry and lymph/blood vessels dynamic microscopy -: art. no. 054018
In vivo integrated flow image cytometry and lymph/blood vessels dynamic microscopy -: art. no. 054018
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DOI：
10.1117/1.2060567
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发表时间：
2005-09-01
影响因子：
3.5
通讯作者：
Zharov, VP
中科院分区：
文献类型：
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作者：
Galanzha, EI;Tuchin, VV;Zharov, VP
利用高空间分辨率（350 nm）透射数字显微镜（TDM），在无造影剂的条件下，对大鼠肠系膜微血管进行单细胞水平的真实的活体成像。肠系膜的主要显微结构（淋巴管直径、瓣膜几何形状、细胞等）以及它们的动力学（室壁运动、瓣膜功能、细胞速度等）。采用TDM监测。根据结构尺寸，使用不同的放大倍数对相对较大的整个淋巴管（X4至X10）以及淋巴和血流中的单个细胞（X40至X100）进行成像，包括估计其形状、尺寸和聚集状态。TDM在体内研究的各种潜在的应用进行了讨论，包括可视化的淋巴和血液流动中的循环细胞，研究血小板的动力学，白细胞滚动，以及成像吸收非荧光肠系膜结构和白细胞具有高的光学分辨率。(c)2005年，由光学仪器工程师学会（Society of Photo-Optical Instrumentation Engineers）主办。
The high spatial resolution (similar to 350 nm) transmission digital microscopy (TDM) was developed for real time in vivo imaging of microlymphatics of rat mesentery at a single cell level without any contrast agent. The main mesenteric microstructures (lymph-vessel diameter, valve geometry, cells, etc.) and their dynamics (wall motion, valve function, cell velocity, etc.) were monitored with TDM. Depending on structure size, different magnifications were used to image relatively large whole lymphangion (X4 to X10) as well as to image single cells (X40 to X100) in lymph and blood flow including estimation of their shape, size, and aggregation state. Various potential applications of the TDM for in vivo studies are discussed, including visualization of circulating cells in lymph and blood flows, studying the kinetics of platelets, leukocyte rolling, as well as imaging absorbing nonfluorescent mesentery structures and leukocytes with a high optical resolution. (c) 2005 Society of Photo-Optical Instrumentation Engineers.