Size-Dependent Deposition, Translocation, and Microglial Activation of Inhaled Silver Nanoparticles in the Rodent Nose and Brain.
Size-Dependent Deposition, Translocation, and Microglial Activation of Inhaled Silver Nanoparticles in the Rodent Nose and Brain.
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DOI：
10.1289/ehp234
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发表时间：
2016-12
影响因子：
10.4
通讯作者：
Pinkerton KE
中科院分区：
文献类型：
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作者：
Patchin ES;Anderson DS;Silva RM;Uyeminami DL;Scott GM;Guo T;Van Winkle LS;Pinkerton KE
银纳米颗粒(AgNP)存在于个人、商业和工业产品中，这些产品通常是雾化的。目前对AgNP吸入的沉积、移位和与健康相关的影响的了解有限。我们确定了a)吸入的银在鼻腔内的沉积和滞留；b)银转移到嗅球(OB)的时间和在OB中的滞留/清除；以及c)OB中银的存在是否影响小胶质细胞的活动。雄性SD大鼠鼻部暴露于含柠檬酸缓冲液的20 nm或110 nm AgNP(分别为C20或C110)或仅含柠檬酸缓冲液的AgNP 6小时。在暴露后56天(8周)，检查鼻腔和OB中银的存在和生物学反应。在第0天观察到两种AgNP大小的鼻部Ag沉积最高。雾化吸入C20后，0、1、7天小胶质细胞的激活和Ag迅速转移到OB，而吸入C110则导致Ag逐渐转移到OB并滞留在OB，小胶质细胞的激活有不同程度的高于对照组的趋势。这项研究的结果表明，在大鼠在单一时间点吸入20 nm和110 nm AgNP 6小时后，鼻子中的Ag沉积、转位到大脑的速率以及随后OB中的小胶质细胞激活情况因AgNP的大小和暴露后的时间而有所不同。Patches，Anderson DS，Silva RM，Uyinami DL，Scott GM，Guo T，Van Winkle LS，Pinkerton Ke。2016年。吸入的银纳米颗粒在啮齿动物鼻子和脑中的大小依赖沉积、移位和小胶质细胞激活。环境健康展望124:1870–1875； http://dx.doi.org/10.1289/EHP234
Silver nanoparticles (AgNP) are present in personal, commercial, and industrial products, which are often aerosolized. Current understanding of the deposition, translocation, and health-related impacts of AgNP inhalation is limited. We determined a) the deposition and retention of inhaled Ag in the nasal cavity from nose-only exposure; b) the timing for Ag translocation to and retention/clearance in the olfactory bulb (OB); and c) whether the presence of Ag in the OB affects microglial activity. Male Sprague-Dawley rats were exposed nose-only to citrate-buffered 20- or 110-nm AgNP (C20 or C110, respectively) or citrate buffer alone for 6 hr. The nasal cavity and OB were examined for the presence of Ag and for biological responses up to 56 days post-exposure (8 weeks). The highest nasal Ag deposition was observed on Day 0 for both AgNP sizes. Inhalation of aerosolized C20 resulted in rapid translocation of Ag to the OB and in microglial activation at Days 0, 1, and 7. In contrast, inhalation of C110 resulted in a gradual but progressive transport of Ag to and retention in the OB, with a trend for microglial activation to variably be above control. The results of this study show that after rats experienced a 6-hr inhalation exposure to 20- and 110-nm AgNP at a single point in time, Ag deposition in the nose, the rate of translocation to the brain, and subsequent microglial activation in the OB differed depending on AgNP size and time since exposure. Patchin ES, Anderson DS, Silva RM, Uyeminami DL, Scott GM, Guo T, Van Winkle LS, Pinkerton KE. 2016. Size-dependent deposition, translocation, and microglial activation of inhaled silver nanoparticles in the rodent nose and brain. Environ Health Perspect 124:1870–1875; http://dx.doi.org/10.1289/EHP234