Wound dressing composed of hyaluronic acid and collagen containing EGF or bFGF: comparative culture study
Wound dressing composed of hyaluronic acid and collagen containing EGF or bFGF: comparative culture study
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DOI：
10.1080/09205063.2012.731375
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发表时间：
2013-05
期刊：
影响因子：
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通讯作者：
Akane Yu;Hayato Niiyama;Shinya Kondo;A. Yamamoto;Ryusuke Suzuki;Y. Kuroyanagi
中科院分区：
文献类型：
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作者：
Akane Yu;Hayato Niiyama;Shinya Kondo;A. Yamamoto;Ryusuke Suzuki;Y. Kuroyanagi
我们通过冷冻干燥方法开发了一种由含有表皮生长因子（EGF）或碱性成纤维细胞生长因子（bFGF）的透明质酸（HA）和胶原（Col）海绵片组成的新型伤口敷料（分别为EGF伤口敷料或bFGF伤口敷料）。以与上述类似的方式制备不含任何生长因子的伤口敷料作为对照（C-伤口敷料）。Col分子之间的分子间交联由UV照射诱导。使用C-伤口敷料研究了伤口敷料中游离HA的释放行为。在空气-水界面处的Col凝胶片上孵育1天、3天、5天和7天后，C-伤口敷料的重量分别为初始重量的55%、36%、30%和19%。大部分游离HA和一部分Col在孵育期间从伤口敷料中的交联Col网络中释放，因为伤口敷料中的原始Col含量为33%。接下来，在通过浸没一片C-、EGF-或bFGF-伤口敷料预处理的常规培养基（即C-条件培养基、EGF-条件培养基或bFGF-条件培养基）中评估成纤维细胞增殖。在C-条件培养基中的细胞增殖增加到与常规培养基中的细胞增殖大致相同的水平。EGF和bFGF条件培养基中的细胞增殖是1.9倍和2.6倍以上，在传统的培养基中培养7天后，分别。最后，在伤口表面模型中使用成纤维细胞掺入的Col凝胶片（培养的真皮替代物[CDS]）评估成纤维细胞的细胞因子产生。将CDS升高至空气-介质界面，在其上放置每种伤口敷料并培养7天。与C型伤口敷料相比，EGF伤口敷料覆盖的CDS中的成纤维细胞释放的血管内皮生长因子（VEGF）和肝细胞生长因子（HGF）分别是C型伤口敷料的3.6倍和4.6倍。与C型伤口敷料相比，bFGF伤口敷料覆盖的CDS中的成纤维细胞释放的VEGF是C型伤口敷料的10.2倍，HGF是C型伤口敷料的6.3倍。这一发现表明，与EGF伤口敷料相比，bFGF伤口敷料可以更有效地促进VEGF和FGF的产生。
We developed a novel wound dressing composed of a hyaluronic acid (HA) and collagen (Col) spongy sheet containing epidermal growth factor (EGF) or basic fibrolast growth factor (bFGF) by freeze-drying method (EGF-wound dressing or bFGF-wound dressing, respectively). A wound dressing without any growth factor was prepared as a control in a similar manner as above (C-wound dressing). Intermolecular cross-linkage between Col molecules was induced by UV irradiation. The release behavior of free HA from the wound dressing was investigated using a C-wound dressing. The weight of C-wound dressing after 1 day, 3, 5, and 7 days of incubation on top of a Col gel sheet at the air–water interface (wound surface model) was 55, 36, 30, and 19% of the original weight, respectively. Most free HA and a part of Col was released from the cross-linked Col network in the wound dressing during incubation, as the original Col content in the wound dressing was 33%. Next, fibroblast proliferation was assessed in conventional culture medium preconditioned by immersion of a piece of C-, EGF-, or bFGF-wound dressing, i.e. C-conditioned medium, EGF-conditioned medium, or bFGF-conditioned medium. Cell proliferation in C-conditioned medium increased to approximately the same level as that in conventional medium. Cell proliferation in EGF- and bFGF-conditioned medium was 1.9 times and 2.6 times greater than that in conventional medium after 7 days of cultivation, respectively. Finally, cytokine production of fibroblasts was assessed in a wound surface model using a fibroblast-incorporating Col gel sheet (cultured dermal substitute [CDS]). CDS was elevated to the air–medium interface, on which each wound dressing was placed and cultured for 7 days. Fibroblasts in CDS covered with EGF-wound dressing released 3.6 times more vascular endothelial growth factor (VEGF) and 4.6 times more hepatocyte growth factor (HGF) when compared with the C-wound dressing. Fibroblasts in CDS covered with bFGF-wound dressing released 10.2 times more VEGF and 6.3 times more HGF when compared with the C-wound dressing. This finding indicates that bFGF-wound dressing can facilitate more effectively the VEGF and FGF production compared with EGF-wound dressing.