Remodeling of neuronal membranes as an early response to deafferentation. A freeze-fracture study.
Remodeling of neuronal membranes as an early response to deafferentation. A freeze-fracture study.
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DOI：
10.1083/jcb.75.3.837
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发表时间：
1977-12
期刊：
影响因子：
--
通讯作者：
Neises GR
中科院分区：
文献类型：
--
作者：
Gulley RL;Wenthold RJ;Neises GR
采用冷冻骨折技术研究了传入神经阻滞对前腹侧耳蜗核（AVCN）Held 终球下方突触后膜的早期影响。耳蜗消融后，在外膜小叶上观察到三种不同的反应。在 12 小时内，通过向膜中添加新颗粒，非聚集颗粒的数量增加了 147%。非聚集颗粒数量的增加持续到耳蜗消融后 4 天。其他反应稍后发生，在末端球茎出现退行性变化之后。耳蜗消融后 1 至 2 天，突触后活动区周围的突触周围聚集体数量减少至正常数量的 10%。 4 天后，所有突触周围聚集物均从膜上消失。包被的囊泡可能参与去除这些聚集物。在 1 到 3 天之间，连接聚集体的数量减少，但聚集体的大小增加，显然是由于附近连接聚集体合并的结果。耳蜗消融后的前 6 天内，膜中连接聚集体中的颗粒总数没有改变。这三个独立的反应表明在主细胞膜的外部小叶上存在至少三种不同类型的膜内颗粒，每种类型依赖于不同的线索来维持其在膜中。
The early effects of deafferentation on the postsynaptic membrane beneath the end bulb of Held in the anteroventral cochlear nucleus (AVCN) were studied with the freeze-fracture technique. Three distinct responses were seen on the external membrane leaflet after cochlear ablation. Within 12 h the number of nonaggregate particles increased 147% by the addition of new particles to the membrane. The increase in number of nonaggregate particles continued until 4 days after cochlear ablation. The other responses occurred later, after degenerative changes were present in the end bulb. Between 1 and 2 days after cochlear ablation, the number of perisynaptic aggregates surrounding the postsynaptic active zone decreased to 10% of normal numbers. By 4 days, all perisynaptic aggregates had disappeared from the membrane. Coated vesicles may be involved in removing these aggregates. Between 1 and 3 days, the number of junctional aggregates decreased, but the size of the aggregates increased, apparently as a result of coalescence of nearby junctional aggregates. The total number of particles in junctional aggregates in the membrane was not altered during the first 6 days after cochlear ablation. The three separate responses suggest the existence of at least three different types of intramembranous particles on the external leaflet of the principal cell membrane, with each type dependent upon different cues for its maintenance in the membrane.