Evaluating the glucose tolerance test in mice
Evaluating the glucose tolerance test in mice
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DOI：
10.1152/ajpendo.90617.2008
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发表时间：
2008-12-01
影响因子：
5.1
通讯作者：
Proietto, Joseph
中科院分区：
文献类型：
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作者：
Andrikopoulos, Sofianos;Blair, Amy R.;Proietto, Joseph
Andrikopoulos S，Blair AR，Deluca N，Fam BC，Proietto J.评价小鼠葡萄糖耐量试验。Am J Physiol Endocrinol Metab 295：E1323-E1332，2008.首次发表于2008年9月23日; doi：10.1152/ajpendo.90617.2008。- 本研究的目的是确定评估普通饲料和高脂饲料喂养的C57 BL/6 J小鼠葡萄糖耐量的最佳条件。给小鼠喂普通饲料或高脂饲料8周。检测的变量为空腹持续时间（0、3、6和24小时以及空腹过夜）、给药途径（腹膜内vs.口服）葡萄糖负荷（2、1或0.5 g/kg和固定50 mg剂量）和意识状态。禁食6小时后，与普通饲料喂养的小鼠相比，高脂肪喂养的小鼠的基础葡萄糖浓度增加（9.1 +/- 0.3 vs. 7.9 +/- 0.4 mmol/l P = 0.01）。在禁食6小时后，高脂喂养小鼠的葡萄糖耐量差异最大，因此具有显著性（P = 0.001）（1，973 +/- 96 vs. 1，248 +/- 83 mmol.l（-1. 120 min（-1）。OGTT后葡萄糖耐量的差异（142%）大于IPGTT，高脂肪和普通食物之间的差异为127%。我们还发现，与普通饲料喂养的小鼠相比，给予2 g/kg葡萄糖导致高脂肪小鼠葡萄糖耐受不良的显著性水平更高（P = 0.0008）。无论体重如何，50 mg葡萄糖的固定剂量足以在高脂肪与普通饲料喂养的小鼠中显示葡萄糖耐受不良。最后，无论是在清醒状态下还是在麻醉状态下，高脂喂养的小鼠与普通食物喂养的小鼠相比，都表现出葡萄糖耐受不良。我们得出结论，2 g/kg葡萄糖口服给药后6小时禁食是最好的评估在这些条件下的小鼠的葡萄糖耐量。
Andrikopoulos S, Blair AR, Deluca N, Fam BC, Proietto J. Evaluating the glucose tolerance test in mice. Am J Physiol Endocrinol Metab 295: E1323-E1332, 2008. First published September 23, 2008; doi: 10.1152/ajpendo.90617.2008. - The objective of this study was to determine the optimal conditions under which to assess glucose tolerance in chow- and high-fat-fed C57BL/6J mice. Mice were fed either chow or high-fat diet for 8 wk. Variables tested were fasting duration (0-, 3-, 6-, and 24-h and overnight fasting), route of administration (intraperitoneal vs. oral) load of glucose given (2, 1, or 0.5 g/kg and fixed 50-mg dose), and state of consciousness. Basal glucose concentrations were increased in high-fat- compared with chow- fed mice following 6 h of fasting (9.1 +/- 0.3 vs. 7.9 +/- 0.4 mmol/l P = 0.01). Glucose tolerance was most different and therefore significant (P = 0.001) in high-fat- fed mice after 6 h of fasting (1,973 +/- 96 vs. 1,248 +/- 83 mmol.l(-1).120 min(-1)). The difference in glucose tolerance was greater following an OGTT (142%), in contrast to an IPGTT, with a 127% difference between high fat and chow. We also found that administering 2 g/kg of glucose resulted in a greater level of significance (P = 0.0008) in glucose intolerance in high-fat-compared with chow- fed mice. A fixed dose of 50 mg glucose regardless of body weight was enough to show glucose intolerance in high-fat- vs. chow- fed mice. Finally, high-fat- fed mice showed glucose intolerance compared with their chow- fed counterparts whether they were tested under conscious or anesthetized conditions. We conclude that 2 g/kg glucose administered orally following 6 h of fasting is best to assess glucose tolerance in mice under these conditions.