Identification of quantitative trait loci affecting shank length, body weight and carcass weight from the Japanese cockfighting chicken breed, Oh-Shamo (Japanese Large Game)
Identification of quantitative trait loci affecting shank length, body weight and carcass weight from the Japanese cockfighting chicken breed, Oh-Shamo (Japanese Large Game)
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DOI：
10.1159/000103190
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发表时间：
2007-07
影响因子：
1.7
通讯作者：
M. Tsudzuki;S. Onitsuka;R. Akiyama;M. Iwamizu;N. Goto;M. Nishibori;H. Takahashi;A. Ishikawa
中科院分区：
文献类型：
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作者：
M. Tsudzuki;S. Onitsuka;R. Akiyama;M. Iwamizu;N. Goto;M. Nishibori;H. Takahashi;A. Ishikawa
本研究对245只F2鸡资源家族中控制鸡腿长度、体重和胴体重量的QTL进行了定量性状位点（QTL）分析。该资源家族由日本大型斗鸡、Oh-Shamo（日本大型野鸡）和White Leghorn鸡杂交而成。区间定位发现1、4和24号染色体上有3个显著的柄长QTL，在5%的实验水平上，27号染色体上有一个提示的柄长QTL，在10%的实验水平上。在4号染色体和24号染色体上分别鉴定出两个qtl，一个为显著qtl，另一个为提示qtl。正如预期的那样，胴体重qtl与体重高度相关（r = 0.95），在检测到的体重qtl的染色体位置相同。有趣的是，含有这些体重和胴体重qtl的染色体位置与检测到的四个柄长qtl中的两个一致。未发现任何性状具有上位互作效应的QTL。所有检测到的qtl对遗传变异的贡献率分别为小腿长度、体重和胴体重量的98.4%、27.0%和25.9%，表明大多数小腿长度qtl已被鉴定，但由于本文使用的88个微卫星标记覆盖率较低（约占全基因组的46%），许多体重和胴体重量qtl被本分析忽略。
We performed a quantitative trait locus (QTL) analysis to map QTLs controlling shank length, body weight, and carcass weight in a resource family of 245 F2 birds developed from a cross of the large-sized, native, Japanese cockfighting breed, Oh-Shamo (Japanese Large Game), and the White Leghorn breed of chickens. Interval mapping revealed three significant QTLs for shank length on chromosomes 1, 4 and 24 at the experiment-wise 5% level, and a suggestive shank length QTL on chromosome 27 at the experiment-wise 10% level. For body weight two QTLs, one significant and the other suggestive, were identified on chromosomes 4 and 24, respectively. As expected, QTLs for carcass weight, which was highly correlated with body weight (r = 0.95), were detected at the same chromosomal locations as the detected body weight QTLs. Interestingly, the chromosomal locations containing these body weight and carcass weight QTLs coincided with those of two of the four shank length QTLs detected. No QTL with an epistatic interaction effect was discovered for any trait. The total contribution of all detected QTLs to genetic variance was 98.4%, 27.0% and 25.9% for shank length, body weight and carcass weight, respectively, indicating that most shank length QTLs have been identified but many body weight and carcass weight QTLs have been overlooked by the present analysis because of a low coverage rate of the 88 microsatellite markers used here (approximately 46% of the whole genome).