In vivo glucose monitoring: the clinical reality and the promise
In vivo glucose monitoring: the clinical reality and the promise
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DOI：
10.1016/j.bios.2004.08.016
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发表时间：
2005-04-15
影响因子：
12.6
通讯作者：
Sachedina, N
中科院分区：
文献类型：
--
作者：
Pickup, JC;Hussain, F;Sachedina, N
血糖监测是现代糖尿病管理的重要组成部分。目前有三种体内葡萄糖传感器可用于临床用途：皮下植入安培酶电极、反向离子电渗系统和基于微透析的装置。继续寻求葡萄糖感测技术的改进，例如有线酶技术、粘度亲和感测和完全植入的葡萄糖传感器。非侵入性葡萄糖感测是葡萄糖监测的最终目标，但是研究最多的方法，近红外（NIR）光谱，目前对于临床应用来说太不精确。基于荧光的葡萄糖传感提供了几个优点，我们正在研究的策略，其中包括基于NIR的荧光共振能量转移使用刀豆球蛋白A/葡聚糖;在溶胶-凝胶封装的己糖激酶的固有荧光的变化;和非侵入性葡萄糖监测细胞通过测量葡萄糖相关的变化NADP（H）。(c)2004 Elsevier B.V.保留所有权利。
Glucose monitoring is an essential component of modem diabetes management. Three in vivo glucose sensors are now available for clinical use: a subcutaneously implanted amperometric enzyme electrode, a reverse iontophoresis system and a microdialysis-based device. Improvements in glucose-sensing technology continue to be sought, e.g. wired enzyme technology, viscometric affinity sensing and totally implanted glucose sensors. Non-invasive glucose sensing is the ultimate goal of glucose monitoring, but the most investigated approach, nearinfrared (NIR) spectroscopy, is presently too imprecise for clinical application. Fluorescence-based glucose sensing offers several advantages and we are investigating strategies which include NIR-based fluorescence resonance energy transfer using concanavalin A/dextran; changes in the intrinsic fluorescence of hexokinase encapsulated in sol-gel; and non-invasive glucose monitoring of cells by measuring glucose-related changes in NADP(H). (c) 2004 Elsevier B.V. All rights reserved.