Estrogen synthetase stimulation by hemin in human choriocarcinoma cell culture.
Estrogen synthetase stimulation by hemin in human choriocarcinoma cell culture.
复制标题
在人绒毛膜癌细胞培养物中血红素刺激雌激素合成酶。
DOI：
10.1016/s0006-291x(85)80149-2
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发表时间：
1985
影响因子：
3.1
通讯作者：
Hussa,RO
中科院分区：
文献类型：
--
作者：
Bellino,FL;Hussa,RO
研究了血红素在培养的人滋养细胞和细胞匀浆中刺激雌激素合成酶（芳香化酶）的能力，并与二丁基cAMP对芳香化酶的刺激进行了比较[(Bu)2cAMP]。在25 μM和0.1 μM浓度下，与hemin一起培养24 h的细胞，或与hemin一起培养45 min的匀浆，分别表现出最大的芳香酶刺激（无hemin时活性为150 ~ 200%）。25 μM血红素可在4 h内刺激芳香酶，而（Bu）2cAMP则需要6 h以上。（Bu）2cAMP培养96 h后，细胞内血红素和卟啉水平比对照细胞高160 ~ 185%。
The ability of hemin to stimulate estrogen synthetase (aromatase) in cultured human trophoblast cells and in cellular homogenates was investigated and compared with aromatase stimulation by dibutyryl cAMP [(Bu)2cAMP]. Cells grown with hemin for 24 h, or homogenates incubated for 45 min with hemin, showed maximal aromatase stimulation (150 to 200% of activities in the absence of hemin) at 25 μM and 0.1 μM, respectively. Aromatase stimulation in culture by 25 μM hemin was observed within 4 h after hemin addition, while (Bu)2cAMP required more than 6 h. Intracellular heme and porphyrin levels were higher (160 to 185%) in 96 h (Bu)2cAMP-grown cells than control cells.