EXPERIMENTAL-ANALYSIS OF HYDROGEN-ION DIFFUSION IN GASTROINTESTINAL MUCUS GLYCOPROTEIN
EXPERIMENTAL-ANALYSIS OF HYDROGEN-ION DIFFUSION IN GASTROINTESTINAL MUCUS GLYCOPROTEIN
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DOI：
10.1152/ajpgi.1981.240.2.g176
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发表时间：
1981-01-01
影响因子：
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通讯作者：
PFEIFFER, CJ
中科院分区：
文献类型：
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作者：
PFEIFFER, CJ
假设粘液糖蛋白阻碍H+扩散，胃粘液的一种可能的保护作用，进行了测试。在设计用于本试验的2个装置（线性扩散室和H+通量室）中评价粘液和粘液糖蛋白（可见粘液、粗粘蛋白、猪、大鼠、兔和人粘液糖蛋白组分）。线性扩散室评估从点源通过粘液糖蛋白到H+检测器的离子扩散。扩散的数学模型，菲克定律的基础上，适用于表征扩散在这个房间。H+通量室通过受控量的粘液糖蛋白评估了跨越陡峭浓度梯度的H+通量率。除人磺粘蛋白外，仅在稀释浓度下和仅在通量室中进行检测，两种程序均表明粘液物质以剂量相关方式阻滞H+扩散。用粗制猪胃粘蛋白进行缓冲的实验在生理条件下没有显示出显著的缓冲作用。目前的实验数据，其原则应外推到粘膜屏障在生物水平上，表明粘液糖蛋白可以抑制H+扩散。
The hypothesis that mucus glycoproteins retard H+ diffusion, a possible protective action of gastric mucus, was tested. Mucus and mucus glycoproteins (visible mucus, crude mucin, mucus glycoprotein fractions from porcine, rat, rabbit and human) were evaluated in 2 devices designed for this test, a linear diffusion chamber and a H+ flux chamber. The linear diffusion chamber assessed ion diffusion from a point source through mucus glycoproteins to a H+ detector. A mathematical model of diffusion, based on Fick''s law, was adapted to characterize diffusion in this chamber. The H+ flux chamber assessed H+ flux rates across a steep concentration gradient through controlled amounts of mucus glycoprotein. With the exception of human sulfomucin, tested only at dilute concentrations and only in the flux chamber, both procedures indicated that mucosubstances retarded, in a dose-related manner, diffusion of H+. Experiments on buffering by crude porcine gastric mucin did not demonstrate significant buffering under physiological conditions. The present experimental data, the principles of which should be extrapolative to the mucous barrier at the biological level, suggest that mucus glycoproteins can inhibit H+ diffusion.