Mixing behavior of 10-(perfluorohexyl)-decanol and DPPC.
Mixing behavior of 10-(perfluorohexyl)-decanol and DPPC.
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10-(全氟己基)-癸醇和 DPPC 的混合行为。
DOI：
10.1016/j.colsurfb.2005.05.014
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Bummer,PaulM
中科院分区：
文献类型：
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作者：
Lehmler,Hans-Joachim;Bummer,PaulM
氟代烃醇如10-（全氟己基）-癸醇对于新型肺部药物递送方法是令人感兴趣的。本研究的目的是调查10-（全氟己基）-癸醇与二棕榈酰磷脂酰胆碱（DPPC），肺表面活性剂的主要成分作为评估有用性的援助，这和其他生物医学应用的混合行为。用差示扫描量热法（DSC）研究了10-（全氟己基）-癸醇对DPPC双层膜（用0.15M氯化钠溶液完全水合）相变的影响。未观察到对应于过量乙醇的峰。氟代醇使DPPC峰展宽，特别是在XDPPC<0.95以下，并消除了XDPPC ≥ 0.91处的DPPC预跃迁。主相转变的开始保持恒定，低至XDPPC = 0.91，表明在凝胶相中的溶解性有限。用氯化钙（2 mM）代替氯化钠水合10-（全氟己基）-癸醇-DPPC混合物不改变宏观相行为。除了热性能，在空气-水界面处的单层中的DPPC中的10-（全氟己基）-癸醇的溶解性在水、氯化钠（0.15M）、氯化钙（2 mM）或盐酸（pH 1.9）亚相中进行了研究。DPPC的液体膨胀到液体冷凝相变的起始压力的浓度依赖性表现出轻微的变化与所有四个亚相的摩尔分数的增加。10-（全氟己基）-癸醇和DPPC在水、氯化钠和氯化钙上的比表面积-摩尔分数图显示出接近理想的行为，在较高的表面压力下有轻微的负偏差。对于盐酸亚相，观察到更显著的负偏差。总体而言，DSC和单层研究表明，10-（全氟己基）-癸醇和DPPC在生物单层和双层中部分混溶。10-（全氟己基）-癸醇-DPPC系统的宏观相行为与类似的烃系统显著不同，这归因于部分氟化的疏水尾部在单层和双层中的不太有利的堆积。
Fluorocarbon alcohol such as 10-(perfluorohexyl)-decanol are of interest for novel pulmonary drug delivery approaches. The purpose of this study was to investigate the mixing behavior of 10-(perfluorohexyl)-decanol with dipalmitoylphosphatidylcholine (DPPC), the major component of lung surfactant as an aid in assessing usefulness for this and other biomedical applications. The impact of 10-(perfluorohexyl)-decanol on the phase transitions of DPPC bilayers fully hydrated with a 0.15M sodium chloride solution were studied using differential scanning calorimetry (DSC). No peak corresponding to excess alcohol was observed. The fluorinated alcohol caused DPPC peak broadening, especially below XDPPC<0.95, and elimination of the pretransition of DPPC at XDPPC∼0.91. The onset of the main phase transition remains constant down to XDPPC∼0.91, suggesting limited miscibility in the gel phase. Hydration of the 10-(perfluorohexyl)-decanol–DPPC mixtures with calcium chloride (2mM) in place of sodium chloride did not alter the macroscopic phase behavior. In addition to the thermal properties, the miscibility of 10-(perfluorohexyl)-decanol in DPPC in monolayers at the air water interface was investigated on water, sodium chloride (0.15M), calcium chloride (2mM) or hydrochloric acid (pH 1.9) subphases. The concentration dependence of the onset pressure of the liquid-expanded to liquid condensed phase transition of DPPC showed a slight change with increasing mole fraction on all four subphases. The surface area-mole fraction diagrams of 10-(perfluorohexyl)-decanol and DPPC on water, sodium chloride and calcium chloride showed near ideal behavior with slight negative deviations at higher surface pressure. A more significant negative deviation was observed for the hydrochloric acid subphase. Overall, both the DSC and the monolayer studies suggest that 10-(perfluorohexyl)-decanol and DPPC are partially miscible in biological mono- and bilayers. The macroscopic phase behavior 10-(perfluorohexyl)-decanol–DPPC system is significantly different from the analogous hydrocarbon system, which is attributed to a less favorable packing of the partially fluorinated hydrophobic tails in the mono- and bilayer.