An electrochemical impedance immunosensor with signal amplification based on Au-colloid labeled antibody complex
An electrochemical impedance immunosensor with signal amplification based on Au-colloid labeled antibody complex
复制标题
基于金胶体标记抗体复合物的信号放大电化学阻抗免疫传感器
DOI：
10.1016/j.snb.2005.11.026
复制
发表时间：
2006-09-12
影响因子：
8.4
通讯作者：
Yu, Ru-Qin
中科院分区：
文献类型：
--
作者：
Chen, Huan;Jiang, Jian-Hui;Yu, Ru-Qin
采用新型扩增程序开发了一种高灵敏度电化学阻抗免疫传感器，其中应用金胶体标记抗体作为初级放大探针，并通过用金胶体标记二抗和金胶体标记抗体交替处理所得组件进行多步扩增。兔抗人 IgG 抗体通过自组装胶体 An 层固定在金电极上。使用兔抗人 IgG 抗体修饰的传感界面，通过阻抗测量来检测分析物人 IgG。在初级放大中，使用An-胶体标记的山羊抗人IgG抗体来放大抗原与免疫传感器表面结合所产生的电子转移电阻。通过金胶体标记的兔抗山羊IgG抗体和金胶体标记的山羊抗人IgG抗体的顺序结合进行进一步的扩增。观察到阻抗增量与 15.3-328.3 ng L-1 范围内的 IgG 浓度呈线性相关，检测限为 4.1 ng L-1。 (c) 2005 Elsevier B.V. 保留所有权利。
A highly sensitive electrochemical impedance immunosensor was developed by using a novel amplification procedure with the application of a Au-colloid labeled antibody as the primary amplifying probe and a multistep amplification by alternating treatment of the resulting assembly with a Au-colloid labeled secondary antibody and a Au-colloid labeled antibody. The rabbit anti-human IgG antibody was immobilized through a selfassembled colloidal An layer on the gold electrode. The analyte, human IgG, was detected through the impedance measurements with the sensing interface modified by the rabbit anti-human IgG antibody. In the primary amplification, the An-colloid labeled goat anti-human IgG antibody was used to amplify the electron-transfer resistance resulting from the binding of the antigen to the immunosensor surface. A further amplification was performed through sequential binding of the Au-colloid labeled rabbit anti-goat IgG antibody and the Au-colloid labeled goat anti-human IgG antibody. The impedance increments were observed to be linearly dependent upon the IgG concentration in the range of 15.3-328.3 ng L-1 with a detection limit of 4.1 ng L-1. (c) 2005 Elsevier B.V. All rights reserved.