The Prx Q protein of Arabidopsis thaliana is a member of the luminal chloroplast proteome
The Prx Q protein of Arabidopsis thaliana is a member of the luminal chloroplast proteome
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拟南芥的 Prx Q 蛋白是腔叶绿体蛋白质组的成员
DOI：
10.1016/j.febslet.2006.10.001
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发表时间：
2006
期刊：
影响因子：
3.5
通讯作者：
W. Schröder
中科院分区：
文献类型：
--
作者：
U. Petersson;T. Kieselbach;José G García;W. Schröder
过氧化物酶在从真细菌到哺乳动物的许多生物体中被发现，其已知的生物学功能包括氧化防御和信号转导。拟南芥的基因组编码10个单独的过氧化物酶，其中4个位于叶绿体中。叶绿体过氧化物酶的最佳特征成员是2-Cys Prx，其与类囊体膜的基质侧相关，并被认为参与抗氧化防御和光合作用的保护。这项研究讨论了叶绿体过氧化物氧还蛋白Prx Q，并表明其亚细胞位置是类囊体膜的腔。为了深入了解拟南芥Prx Q蛋白的生物学功能，研究了在不同的光条件和氧化胁迫下类囊体膜中Prx Q蛋白的蛋白水平。Prx Q的T-DNA敲除突变体没有表现出任何可见的表型，并且具有正常的光合性能，具有略微增加的放氧活性。
Peroxiredoxins have been discovered in many organisms ranging from eubacteria to mammals, and their known biological functions include both oxidant defense and signal transduction. The genome of Arabidopsis thaliana encodes for ten individual peroxiredoxins, of which four are located in the chloroplast. The best-characterized member of the chloroplast peroxiredoxins is 2-Cys Prx that is associated with the stroma side of the thylakoid membrane and is considered to participate in antioxidant defense and protection of photosynthesis. This study addressed the chloroplast peroxiredoxin Prx Q and showed that its subcellular location is the lumen of the thylakoid membrane. To get insight in the biological function of the Prx Q protein of Arabidopsis, the protein levels of the Prx Q protein in thylakoid membranes were studied under different light conditions and oxidative stress. A T-DNA knockout mutant of Prx Q did not show any visible phenotype and had normal photosynthetic performance with a slightly increased oxygen evolving activity.