Expression of proinflammatory and Th1 but not Th2 cytokines is enhanced in gastric mucose of Helicobacter pylori infected children
Expression of proinflammatory and Th1 but not Th2 cytokines is enhanced in gastric mucose of Helicobacter pylori infected children
复制标题
DOI：
10.1016/s1590-8658(01)80130-4
复制
发表时间：
2001-01-01
影响因子：
4.5
通讯作者：
Pallone, F
中科院分区：
文献类型：
--
作者：
Luzza, F;Parrello, T;Pallone, F
背景。幽门螺杆菌诱导的胃部炎症被认为在很大程度上受细胞因子调节。
患者与方法。通过半定量逆转录聚合酶链反应和Southern杂交技术检测24名儿童胃黏膜样本中干扰素 -γ、白细胞介素 - 12、白细胞介素 - 4、白细胞介素 - 10、白细胞介素 - 8和白细胞介素 - 17 mRNA的表达。采用基于活检的检测、血清学检测以及尿素C - 13呼气试验来评估幽门螺杆菌感染状况。还对胃活检组织进行细菌密度、慢性炎症以及急性炎症活动的评估。
结果。幽门螺杆菌感染（n = 13）儿童的干扰素 -γ、白细胞介素 - 12、白细胞介素 - 8和白细胞介素 - 17表达高于未感染（n = 11）儿童。相反，白细胞介素 - 4和白细胞介素 - 10在幽门螺杆菌感染和未感染儿童之间的表达无差异。在幽门螺杆菌感染儿童中，干扰素 -γ、白细胞介素 - 12、白细胞介素 - 8和白细胞介素 - 17的表达与细菌密度相关，干扰素 -γ和白细胞介素 - 12的表达与慢性炎症评分相关。
结论。本研究结果表明，在儿童中，幽门螺杆菌诱导的炎症反应有利于促炎细胞因子的产生以及细胞介导免疫（即Th1反应）的发展。
Background. Helicobacter pylori-induced gastric inflammation is thought to be largely regulated by cytokines.Patients and methods. The expression of interferon-gamma, interleukin-12 interleukin-4, interleukin-10, interleukin-8, and interleukin-17 mRNA was examined on gastric mucosal samples from 24 children by semiquantitative reverse transcription polymerase chain reaction and southern blotting. Biopsy-based tests, serology, and urea C-13 breath test were used to assess Helicobacter pylori status. Gastric biopsies were also evaluated for bacterial density, chronic inflammation, and acute inflammatory activityResults. Interferon-gamma, interleukin-12, interleukin-8 and interleukin-17 expression was higher in Helicobacter pylori-infected(n = 13) than uninfected (n = 11) children. Conversely, interleukin-4 and interleukin-10 expression did not differ between Helicobacter pylori-infected and uninfected children. In Helicobacter pylori-infected children, interferon-gamma, interleukin-12, interleukin-8 and interleukin-17 expression correlated with bacterial density, and Interferon-gamma and interleukin-12 expression with chronic inflammation score.Conclusions. The findings of this study indicate that, in children, Helicobacter pylori-induced inflammatory response would favour production of proinflammatory cytokines and development of cell-mediated immunity, namely Th1 response.