HETEROGENEITY OF VIRUS-LIKE GENES ENCODING RETROVIRUS-ASSOCIATED 30S-RNA AND THEIR ORGANIZATION WITHIN THE MOUSE GENOME
HETEROGENEITY OF VIRUS-LIKE GENES ENCODING RETROVIRUS-ASSOCIATED 30S-RNA AND THEIR ORGANIZATION WITHIN THE MOUSE GENOME
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DOI：
10.1016/0092-8674(80)90629-7
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发表时间：
1980-01-01
期刊：
影响因子：
64.5
通讯作者：
AVIV, H
中科院分区：
文献类型：
--
作者：
KESHET, E;SHAUL, Y;AVIV, H
小鼠基因组含有一组重复20-50次的基因，其通常表达30 S RNA转录物。这些转录本可以作为假型特异性地拯救到辅助非依赖性C型病毒的病毒体中，并且被认为是有缺陷的内源性逆转录病毒。从噬菌体中的近交系小鼠品系BALB/c的基因文库中克隆编码病毒样30 S RNA的DNA序列。通过与小鼠细胞产生的MuLV [小鼠白血病病毒]病毒体制备中丰富的RNA杂交，但与MuLV基因组没有序列同源性，以及克隆的DNA与未感染小鼠细胞胞质中含有poly（A）的30 S RNA特异性杂交的能力，鉴定了这些序列。30 S RNA由基因组中不间断的5 kb [DNA酶] DNA序列编码。病毒样基因的单个拷贝分布在不同的位点，两侧是没有明显序列同源性的细胞DNA序列。每一个病毒样基因都与所有其他拷贝相关但不完全相同。检测到两个层次的遗传异质性：第一个结果从相对较大的，连续的序列的非同源性长达300个碱基对;第二个结果从分散的碱基替换的模式表明，一些DNA序列可能比其他的保守。
The mouse genome harbors a set of genes reiterated 20-50 times that normally expresses 30S RNA transcripts. These transcripts can be rescued specifically into virions of helper-independent C-type viruses as pseudotypes and are thought to be defective endogenous retroviruses. DNA sequences coding virus-like 30S RNA were cloned from a gene library of the inbred mouse strain BALB/c in bacteriophage .lambda.. The sequences were identified by their hybridization with an RNA abundant in preparation of MuLV [murine leukemia virus] virions produced by mouse cells but sharing no sequence homology with the MuLV genome, and by the ability of the cloned DNA to hybridize specifically with the poly(A)-containing 30S RNA present in the cytoplasm of uninfected mouse cells. The 30S RNA are encoded by uninterrupted 5 kb [kilobase] DNA sequences in the genome. Individual copies of virus-like genes were distributed at different loci and were flanked by cellular DNA sequences that shared no apparent sequence homology. Each of the virus-like genes is related but not identical to all other copies. Two levels of genetic heterogeneity were detected: the first results from relatively large, continuous sequences of nonhomology up to 300 base pairs long; the second results from a pattern of scattered base substitutions suggesting that some DNA sequences might have been conserved more than others.