NUCLEOTIDE-SEQUENCE OF THE GENOMIC RNA OF BAMBOO MOSAIC POTEXVIRUS
NUCLEOTIDE-SEQUENCE OF THE GENOMIC RNA OF BAMBOO MOSAIC POTEXVIRUS
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DOI：
10.1099/0022-1317-75-9-2513
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发表时间：
1994-09-01
影响因子：
3.8
通讯作者：
HSU, YH
中科院分区：
文献类型：
--
作者：
LIN, NS;LIN, BY;HSU, YH
通过对一组重叠cDNA克隆的测序和对病毒RNA的直接测序，测定了竹花叶病毒（BaMV）基因组RNA的全核苷酸序列。BaMV的RNA基因组长度为6366个核苷酸[不包括3' poly（A）尾]，包含6个开放阅读框（ORF 1至6），编码的多肽的M（r）值分别为155 K、28 K、13 K、6 K、25 K和14 K。基因组结构和编码蛋白的大小与已测序的其他马铃薯X病毒非常相似，除了ORF 6完全位于ORF 1内。BaMV基因组的前五个推定蛋白质与马铃薯X病毒组的其他成员的相应ORF的同一性分别为44 - 59%、26 - 49%、30 - 53%、15 - 35%和20 - 30%。然而，推定产物ORF 6显示与其他马铃薯X病毒ORF产物没有显著的相似性。
The complete nucleotide sequence of the genomic RNA of bamboo mosaic virus (BaMV) was determined by sequencing a set of overlapping cDNA clones and by direct sequencing of the viral RNA. The RNA genome of BaMV is 6366 nucleotides long [excluding 3' poly(A) tail] and contains six open reading frames (ORFs 1 to 6) coding for polypeptides with M(r) values of 155K, 28K, 13K, 6K, 25K and 14K, respectively. The genome organization and sizes of the encoded proteins are very similar to those of other potexviruses which have been sequenced except that ORF 6 lies completely within ORF 1. The first five putative proteins of the BaMV genome show identities ranging between 44 to 59%, 26 to 49%, 30 to 53%, 15 to 35% and 20 to 30%, respectively, to the corresponding ORFs of other members of the potexvirus group. However the putative product ORF 6 shows no significant similarity to those of other potexvirus ORF products.