Identification of novel targets of cyanobacterial glutaredoxin
Identification of novel targets of cyanobacterial glutaredoxin
复制标题
蓝藻谷氧还蛋白新靶标的鉴定
DOI：
10.1016/j.abb.2006.12.010
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发表时间：
2007-02-15
影响因子：
3.9
通讯作者：
Wu, Qingyu
中科院分区：
文献类型：
--
作者：
Li, Min;Yang, Qing;Wu, Qingyu
谷氧还蛋白(Glutaredoxins，Grx)是一种广泛存在的小分子谷胱甘肽-二硫键氧化还原酶，它能还原靶蛋白的二硫键，维持细胞氧化还原平衡。干扰ssr2061使细胞活力降低，表明Grx2061对集胞藻的氧化应激具有保护作用。PCC 6803。为了了解Grx2061在蓝藻中的功能及其与植物的差异，将GRX靶标特异性地保留在与突变的单半胱氨酸GRX偶联的亲和介质上，并通过质谱学进行鉴定。在42个已鉴定的靶点中，有26个是与高等植物中已知的相比是新的靶点。这些蛋白质被认为参与了12个细胞过程，包括氧化应激反应、Calvin循环、蛋白质合成等。生化测试突出了其中4个过程，显示GRX依赖的过氧化还蛋白激活和过氧化氢酶失活。氧化后的Grx2061可以与另一个可能的GRX保持氧化还原平衡，并被硫氧还蛋白还原酶还原，表明Grx2061可以接受谷胱甘肽或硫氧还蛋白还原酶的电子。我们的研究表明，蓝藻中的Grx2061在氧化还原网络中起着重要的作用，其靶标与其他生物一样广泛。(C)2006 Elsevier Inc.保留所有权利。
Glutaredoxins (Grxs) are small ubiquitous glutathione-disulfide oxidoreductase that reduce disulfide bonds of target proteins and maintain the redox homoeostasis of cells. Disruption of ssr2061 reduced the viability of cells indicated Grx2061 has a protective role against oxidative stress in Synechocystis sp. PCC 6803. To understand the function of Grx2061 in cyanobacteria and its difference from plant, Grx targets were retained specifically on an affinity media coupled with a mutated monocysteinic Grx and identified by mass spectra. Among 42 identified targets, 26 of them are novel ones compared with those known in higher plants. These proteins are supposed to be involved in 12 cellular processes including oxidative stress response, Calvin cycle, protein synthesis, and etc. Biochemical tests highlighted four of them which showed a Grx-dependent activation of peroxiredoxin and deactivation of catalase. Oxidized Grx2061 could keep redox equilibrium with another probable Grx and be reduced by thioredoxin reductase, indicating that Grx2061 can accept electrons from either glutathione or thioredoxin reductase. Our studies suggest Grx2061 in cyanobacteria plays an important role in redox network and its targets are as extensive as that in other organisms. (c) 2006 Elsevier Inc. All rights reserved.