In vitro histogenic capacities of limb mesenchyme from various stage mouse embryos.
In vitro histogenic capacities of limb mesenchyme from various stage mouse embryos.
复制标题
不同阶段小鼠胚胎肢体间充质的体外组织形成能力。
DOI：
10.1016/0012-1606(81)90173-1
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发表时间：
1981
影响因子：
2.7
通讯作者：
Solursh,M
中科院分区：
文献类型：
--
作者：
Owens,EM;Solursh,M
间充质细胞分离小鼠胚胎前肢芽（阶段15至21），并放置在高密度micromass文化进行了比较，就theirin vitrohistogenic能力。特别强调的是放在改变invitrochondrogenic能力。第15阶段小鼠肢体培养物形成许多聚集体，其均不能分化成软骨结节。另一方面，在从所有后续阶段肢体制备的培养物中观察到软骨结节，尽管在16-17期和21中晚期培养物之间结节的大小线性减小。这种减少与聚集细胞的比例和二丁酰环AMP刺激的软骨形成的程度同时减少相关。同时，观察表明，非聚集性和非软骨形成间充质，肌源性细胞，也许，纤维形成间充质的比例正在增加。这些模式的唯一例外是在中晚期21肢的培养物中观察到的，此时软骨原位分化已经很广泛。与形成几乎相同数量的聚集体和结节的早期培养物不同，中晚期培养物形成可变数量的聚集体，其中只有少数分化成软骨结节。中晚期21期培养物还含有出乎意料的低数量的肌原性细胞/单位面积培养物。基于thein vitrohistogenic能力的变化，可以得出结论，在肢体中的形态发生事件的进展的同时，有一个进展的细胞亚群的相对比例的变化。不同亚群的存在及其相对比例的变化都可以在体外检测到。此外，它的结论是，软骨形成在小鼠和鸡胚胎的四肢可能发生根据非常相似的发育程序。
Mesenchyme cells isolated from mouse embryo forelimb buds (stages 15 through 21) and placed in high-density micromass cultures are compared with respect to theirin vitrohistogenic capacities. Particular emphasis is placed on changes inin vitrochondrogenic capacity. Stage 15 mouse limb cultures form numerous aggregates which uniformly fail to differentiate into cartilage nodules. On the other hand, cartilage nodules are observed in cultures prepared from all subsequent stage limbs, although there is a linear decrease in the size of nodules between stage 16–17 and middle-late stage 21 cultures. This decrease correlates with simultaneous decreases in both the proportion of aggregating cells and the extent of dibutyryl cyclic AMP-stimulated cartilage formation. At the same time, observations indicate that the proportions of nonaggregating and nonchondrogenic mesenchyme, myogenic cells, and, perhaps, fibrogenic mesenchyme are increasing. The only exceptions to these patterns are observed in cultures from middle-late stage 21 limbs, when cartilage differentiationin situis already extensive. Unlike earlier stage cultures, which form nearly identical numbers of aggregates and nodules, middle-late stage 21 cultures form variable numbers of aggregates, only a few of which differentiate into cartilage nodules. Middle-late stage 21 cultures also contain unexpectedly low numbers of myogenic cells/unit area of culture. Based on changes in thein vitrohistogenic capacities, it is concluded that concurrent with a progression of morphogenic events in the limb, there is a progression of changes in the relative proportions of cell subpopulations. Both the existence of the different subpopulationsandthe changes in their relative proportions can be detectedin vitro. Furthermore, it is concluded that cartilage formation in the limbs of both mouse and chick embryos probably occurs according to very similar developmental programs.