Detection of Actinobacillus actinomycetemcomitans and Bacteroides gingivalis in subgingival smears by the indirect fluorescent-antibody technique.
Detection of Actinobacillus actinomycetemcomitans and Bacteroides gingivalis in subgingival smears by the indirect fluorescent-antibody technique.
复制标题
通过间接荧光抗体技术检测龈下涂片中的伴放线放线杆菌和牙龈拟杆菌。
DOI：
10.1111/j.1600-0765.1985.tb00845.x
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发表时间：
1985
影响因子：
3.5
通讯作者：
G. Dahlén
中科院分区：
文献类型：
--
作者：
J. Slots;C. Hafström;B. Rosling;G. Dahlén
应用间接免疫荧光技术检测伴放线放线杆菌。放线菌共生菌）和牙龈拟杆菌（B.龈下菌斑涂片中的龈下菌斑（gingivalis）。该试验使用了抗A.伴放线菌（3种血清型）和B. gingivalis（1种血清型）和山羊抗兔IgG荧光素缀合物。88个牙周标本的研究，约80%是阳性或阴性的两个物种的文化和免疫荧光，约5%的文化，但不能通过免疫荧光显示的生物体，约13%的文化阴性和免疫荧光阳性。在阳性病变中，免疫荧光倾向于产生较高的A。伴随放线菌计数比培养物和培养物往往产生更高的B。gingivalis计数比免疫荧光。在一项包括21名成年患者和73个深牙周袋的牙周治疗研究中，A。放线菌共生菌或B.免疫荧光法在8个牙周损伤中的5个中检测到牙龈炎，这些牙周损伤在治疗后显示出探测牙周附着的丧失，但在37个治疗的牙周部位中的任何一个中都没有检测到牙龈炎，这些牙周病变显示出探测附着的获得。结论间接免疫荧光法可检测A.放线菌共生菌和B.龈下菌斑中的微生物，监测这些微生物可能有助于区分进展性和非进展性牙周炎病变。
An indirect immunofluorescence technique for the detection of Actinobacillus actinomycetemcomitans (A. actinomycetemcomitans) and Bacteroides gingivalis (B. gingivalis) in smears of subgingival plaque was evaluated. The assay used species-specific rabbit antibodies against A. actinomycetemcomitans (3 serotypes) and B. gingivalis (1 serotype) and a goat anti-rabbit IgG fluorescein conjugate. Of 88 periodontal specimens studied, about 80% were either positive or negative for the two species by both culture and immunofluorescence, about 5% revealed the organisms by culture but not by immunofluorescence, and about 13% were culture-negative and immunofluorescence-positive. In positive lesions, immunofluorescence tended to yield higher A. actinomycetemcomitans counts than culture and culture tended to yield higher B. gingivalis counts than immunofluorescence. In a periodontal treatment study including 21 adult patients and 73 deep periodontal pockets, A. actinomycetemcomitans or B. gingivalis were detected by immunofluorescence in 5 of 8 periodontal lesions which showed loss of probing periodontal attachment after therapy but not in any of 37 treated periodontal sites exhibiting probing attachment gain. It was concluded that indirect immunofluorescence can detect A. actinomycetemcomitans and B. gingivalis in subgingival plaque and that monitoring these organisms may aid in differentiating progressing and nonprogressing periodontitis lesions.