Correlation of Pfg377 ortholog gene expression of Plasmodium vivax and mosquito infection
Correlation of Pfg377 ortholog gene expression of Plasmodium vivax and mosquito infection
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DOI：
10.1111/j.1365-3156.2011.02940.x
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发表时间：
2012-04-01
影响因子：
3.3
通讯作者：
Sattabongkot, J.
中科院分区：
文献类型：
--
作者：
Chansamut, N.;Buates, S.;Sattabongkot, J.
目的检测间日疟原虫Pfg 377同源基因的表达，并探讨其与蚊虫感染的关系。根据卵囊计数确定对雌性大劣按蚊的繁殖力。采用真实的时间反转录-聚合酶链反应（RT-PCR）技术，检测了间日疟原虫Pfg 377同源基因转录产物，结果在70份间日疟原虫阳性血样中，50份（71.4%）为蚊感染，20份（28.6%）为非蚊感染。感染组Pfg 377同源基因的表达水平显著高于非感染组（P < 0.05）。在感染性样品中，Pfg 377同源基因在100拷贝/ ml血液临界点的表达水平与10个卵囊/蚊平均卵囊数的临界点和50%感染蚊的临界点相关（Pearson卡方相关，P = 0.014和P = 0.026，结论削减-Pfg 377同源基因表达水平的临界点可用于预测间日疟原虫配子体的感染性，从而导致蚊子感染和寄生虫传播。领域
Objective To determine the expression of Pfg377 ortholog gene in Plasmodium vivax, and examine its correlation with mosquito infection.methods Seventy clinical blood samples positive for P. vivax by microscopy, were used for the mosquito infectivity assay. Infectivity to female Anopheles dirus was determined from oocyst counts. The transcripts of Pfg377 ortholog gene of P. vivax from blood samples infective and non- infective to mosquitoes were examined using quantitative real- time reverse transcriptase- polymerase chain reaction (RT- PCR).results Of 70 P. vivax positive blood samples, 50 (71.4%) samples were mosquito- infective and 20 (28.6%) were not. In infective samples, the expression level of Pfg377 ortholog gene was significantly higher than in the non- infective group (P < 0.05). In infective samples, the expression level of Pfg377 ortholog gene at 100 copies / ml of blood cut- off point correlated with 10 oocysts / mosquito cut- off point of average oocyst numbers and with 50% cut- off point of per cent infected mosquitoes (Pearson's chi- square correlation, P = 0.014 and P = 0.026, respectively).conclusion The cut- off point of the expression level of Pfg377 ortholog gene could be used to predict the infectiousness of P. vivax gametocytes leading to mosquito infection and parasite transmission in the field.