High-throughput identification of phage-derived imaging agents
High-throughput identification of phage-derived imaging agents
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DOI：
10.2310/7290.2006.00003
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发表时间：
2006-01-01
影响因子：
2.8
通讯作者：
Weissleder, Ralph
中科院分区：
文献类型：
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作者：
Kelly, Kimberly A.;Clemons, Paul A.;Weissleder, Ralph
使用噬菌体显示肽库是选择具有所需结合特性的肽的有力方法。然而，从这种筛选方法中获得的“命中”的验证和优先级仍然具有挑战性。在这里，我们描述。开发和测试一种新的分析方法，以识别和显示来自噬菌体展示实验和高通量酶联免疫吸附试验筛选的命中。我们使用针对活化巨噬细胞的噬菌体筛选来测试该方法，以开发对活动性疾病过程具有更高特异性的显像剂。这种新方法可用于鉴定噬菌体命中，并可扩展到其他文库筛选方法，如小分子和纳米颗粒文库。
The use of phage-displayed peptide libraries is a powerful method for selecting peptides with desired binding properties. However, the validation and prioritization of "hits" obtained from this screening approach remains challenging. Here, we describe the. development and testing of a new analysis method to identify and display hits from phage-display experiments and high-throughput enzyme-linked immunosorbent assay screens. We test the method using a phage screen against activated macrophages to develop imaging agents with higher specificity for active disease processes. The new methodology should be useful in identifying phage hits and is extendable to other library screening methods such as small-molecule and nanoparticle libraries.