Lipid antigens derived from erythrocytes infected withPlasmodium berghei
Lipid antigens derived from erythrocytes infected withPlasmodium berghei
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源自伯氏疟原虫感染的红细胞的脂质抗原
DOI：
10.1007/bf00927819
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
M. Londner
中科院分区：
文献类型：
--
作者：
S. Frankenburg;G. Slutzky;C. Gitler;M. Londner
从感染伯氏疟原虫的红细胞、感染红细胞膜和游离寄生虫中提取脂质。使用放射免疫测定法检测恢复期和免疫大鼠血清中针对这些脂质的抗体。大多数的抗原活性可以归因于寄生虫，虽然一些活动被发现在脂质中分离的膜感染的红细胞。吸收研究表明，结合是特异性的疟疾脂质抗原。免疫血清与未感染大鼠红细胞脂质无交叉反应。然而，来自未感染对照大鼠的血清显示出与寄生的红细胞衍生脂质的低水平交叉反应性。抗脂质抗体的水平与感染的进展直接相关。当寄生虫血症达到零时，抗体水平最高。疟原虫脂质对体外培养的免疫脾细胞的淋巴母细胞转化无影响。然而，从疟疾或非特异性脂质制备的脂质体引起母细胞对抗原的反应增加。
Lipids were extracted from red blood cells infected withPlasmodium berghei, from the membranes of infected red cells and from free parasites. A radioimmunoassay was used to detect antibodies to these lipids in sera from convalescent and immune rats. Most of the antigenic activity could be attributed to the parasite although some activity was found in lipids isolated from the membranes of infected red blood cells. Absorption studies showed that the binding was specific for malarial lipid antigens. Immune sera showed no cross-reactivity with lipids from red blood cells of non-infected rats. However, sera from non-infected control rats showed low levels of cross-reactivity with the parasitized red cell-derived lipids. Levels of anti-lipid antibodies were directly correlated with the progress of the infection. The highest antibody level occurred when the parasitaemia reached zero. The malarial lipids had no effect on lymphoblast transformation of immune splenocytes in vitro. However, liposomes prepared from either malarial or non-specific lipids caused an increased response to antigen by the blast cells.