PRORP proteins support RNase P activity in both organelles and the nucleus in Arabidopsis
PRORP proteins support RNase P activity in both organelles and the nucleus in Arabidopsis
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DOI：
10.1101/gad.189514.112
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发表时间：
2012-05-15
影响因子：
10.5
通讯作者：
Giege, Philippe
中科院分区：
文献类型：
--
作者：
Gutmann, Bernard;Gobert, Anthony;Giege, Philippe
核糖核酸酶P是一种切割tRNA前体59个前导序列的重要酶。到目前为止，人们普遍认为RNaseP以核糖核蛋白的形式存在于具有RNaseP活性的生物体中。在这里，我们发现，只有蛋白质的RNase P酶，称为PRORP(蛋白质RNase P)，在体内支持拟南芥中细胞器和细胞核中的RNase P活性。除tRNA外，PRORP蛋白还参与小核仁RNA(SnoRNA)和mRNA的成熟。最后，核糖核蛋白RNase MRP不参与植物中tRNA的成熟。总之，我们的结果表明，在植物中，核糖核蛋白酶已经完全被RNaseP活性的蛋白质取代。
RNase P is an essential enzyme that cleaves the 59 leader sequence of tRNA precursors. RNase Ps were believed until now to occur universally as ribonucleoproteins in organisms performing RNase P activity. Here we find that protein-only RNase P enzymes called PRORP (for proteinaceous RNase P) support RNase P activity in vivo in both organelles and the nucleus in Arabidopsis. Beyond tRNA, PRORP proteins are involved in the maturation of small nucleolar RNA (snoRNA) and mRNA. Finally, ribonucleoprotein RNase MRP is not involved in tRNA maturation in plants. Altogether, our results indicate that ribonucleoprotein enzymes have been entirely replaced by proteins for RNase P activity in plants.