Label-free detection of human enteric nerve system using Raman spectroscopy: A pilot study for diagnosis of Hirschsprung disease
Label-free detection of human enteric nerve system using Raman spectroscopy: A pilot study for diagnosis of Hirschsprung disease
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DOI：
10.1016/j.jpedsurg.2021.03.040
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发表时间：
2021-06-20
影响因子：
2.4
通讯作者：
Inomata, Masafumi
中科院分区：
文献类型：
--
作者：
Ogawa, Katsuhiro;Oshima, Yusuke;Inomata, Masafumi
背景：先天性巨结肠(HSCR)的特征是缺乏肠道神经系统(ENS)。要去除无神经节细胞增多症，肠重建只是一种根治方法。术中必须确定无神经节细胞增多症的程度。拉曼光谱是一种非破坏性的化学分析技术，它提供了关于分子振动的详细信息。本研究的目的是利用拉曼光谱检测人体肠道中的ENS，以用于诊断HSCR。方法：采集人直肠手术标本的胃肠壁各层的拉曼光谱。基于采集的光谱数据，进行主成分分析来确定ENS。随后，对HSCR截面的拉曼光谱进行了分析。结果：拉曼光谱表征了胃肠壁的分子结构。拉曼光谱可以区分神经节和肌层，无神经节病肌层间边缘的光谱是胶原相关峰。HSCR材料中也证实了ENS的缺失。结论：利用拉曼光谱技术成功地实现了无标记检测。由于这是一项初步研究，可能有助于在HSCR中使用非侵入性技术区分神经节和无神经节段的策略应该在不久的将来通过前瞻性研究进行评估。(C)2021 Elsevier Inc.保留所有权利。
Background: Hirschsprung disease (HSCR) is characterized by the absence of an enteric nerve system (ENS). To remove aganglionosis, bowel reconstruction is only a curative treatment. It is mandatory to identify the extent of aganglionosis during surgery. Raman spectroscopy is a nondestructive chemical analysis technique that provides detailed information regarding molecular vibrations. The purpose of this study is to detect the ENS using Raman spectroscopy in the human intestine for diagnosis of HSCR.& nbsp; Methods: The Raman spectra of each layer of the gastrointestinal wall were collected from surgical specimens of the human rectum. Based on collected spectral data, principal component analysis was performed to determine the ENS. Subsequently, the Raman spectra of HSCR sections were analyzed. Results: Molecular structures of the gastrointestinal wall were characterized by Raman spectroscopy. Raman spectroscopy could discriminate between ganglion and muscle layers, and the spectra of the border between muscle layers in the aganglionosis were collagen-associated peaks. Either absence on presence of ENS was also confirmed in HSCR material. Conclusions: Label-free detection of the ENS was successfully demonstrated using Raman spectroscopy. Since this is a preliminary study, the strategy which may contribute to differentiate between ganglionic and aganglionic segments using noninvasive techniques in HSCR should be evaluated by prospective studies in near future. (c) 2021 Elsevier Inc. All rights reserved.